Co-circulation of all the four Dengue virus serotypes during 2018-2019: first report from Eastern Uttar Pradesh, India

Sthita Pragnya Behera; Pooja Bhardwaj; Hirawati Deval; Neha Srivastava; Rajeev Singh; Brij Ranjan Misra; Awdhesh Agrawal; Asif Kavathekar; Rajni Kant

doi:10.7717/peerj.14504

Co-circulation of all the four Dengue virus serotypes during 2018-2019: first report from Eastern Uttar Pradesh, India

PeerJ. 2023 Jan 9:11:e14504. doi: 10.7717/peerj.14504. eCollection 2023.

Authors

Affiliations

¹ ICMR-Regional Medical Research Centre, Gorakhpur, Uttar Pradesh, India.
² Division of Pathology, Gorakhnath Hospital, Gorakhpur, Uttar Pradesh, India.

^# Contributed equally.

Abstract

Dengue fever is an endemic disease in India, transmitted by an infected mosquito bite. In India, the number of concurrent infections and the circulation of multiple dengue virus (DENV) serotypes has increased in recent decades. Molecular surveillance among the DENV serotype is important to keep track of the circulating serotypes, evolutionary changes, and key mutations that can alter the diagnostics. The current study included patients admitted for dengue in the Eastern Uttar Pradesh (E-UP) region during 2018-2019. The genetic characterization of the circulating DENV was accomplished through partial CprM (511 bp) gene amplification via reverse transcriptase polymerase chain reaction and sequencing. Phylogenetic analysis revealed the circulation of all four DENV1-4 serotypes. DENV-2 was the most abundant serotype (44%, 27/61), followed by DENV-3 (32%, 20/61). DENV-1 had a 16% (10/61) predominance, while DENV-4 (6%, 4/61) was found to be the least abundant serotype. DENV-2 genotypes were distributed among lineages I (7.4%), II (85%) and III (7.4%) of genotype IV, DENV-3 to lineage III of genotype III, DENV-1 to genotype III; DENV-2 to lineage B (75%) and C (25%) of genotype I. This primary report on the co-circulation of DENV1-4 serotypes from the E-UP region highlights the requirement of continuous molecular surveillance for monitoring circulating DENV serotypes.

Keywords: Dengue; E-UP; Genotype; Gorakhpur; Phylogeny; Serotype.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dengue Virus* / genetics
Dengue* / epidemiology
Humans
India / epidemiology
Phylogeny
Serogroup

Grants and funding

This work was supported by the Indian Council of Medical Research, New Delhi (grant number GKP-1504) and the Department of Health Research, HRD, MoHFW, New Delhi, for Young Scientist fellowship scheme (DHR/YSS/000048). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.