Impacts of exogenous quorum sensing signal molecule-acylated homoserine lactones (AHLs) with different addition modes on Anammox process

Xiaojing Zhang; Han Zhang; Nan Zhang; Yongpeng Ma; Nan Liu; Guanglu Han; Qiong Wang

doi:10.1016/j.biortech.2023.128614

Impacts of exogenous quorum sensing signal molecule-acylated homoserine lactones (AHLs) with different addition modes on Anammox process

Bioresour Technol. 2023 Mar:371:128614. doi: 10.1016/j.biortech.2023.128614. Epub 2023 Jan 11.

Authors

Xiaojing Zhang¹, Han Zhang², Nan Zhang², Yongpeng Ma², Nan Liu², Guanglu Han², Qiong Wang²

Affiliations

¹ Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Materials and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China. Electronic address: zhangxiaojing@zzuli.edu.cn.
² Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Materials and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China.

PMID: 36640821
DOI: 10.1016/j.biortech.2023.128614

Abstract

Anammox was proved having the quorum sensing ability, and several acylated homoserine lactones (AHLs) signal molecules were detected in the system. In this study, the impact of exogenous N-dodecanoyl homoserine lactone (C12-HSL) with different addition modes on the nitrogen removal, key enzymes' activity, and microbial revolution were investigated in Anammox system. Results showed that once-addition of C12-HSL had no obvious impact on Anammox. Daily-addition with 40 nM slightly improved the TN removal from 71.1 % to 74.5 %, while 80 and 200 nM significantly decreased it to 62.7 % and 61.8 %, respectively. The enzyme activity of ammonia monooxygenase increased from 0.015 to 0.068, nitrite reductase increased from 0.25 to 1.23, and nitrate reductase increased from 0.05 to 0.11 μg NO₂^--N mg^-1 Protein min^-1. Arenimonas abundance showed positive correlation with TN removal while Candidatus Kuenenia was continuously suppressed. C12-HSL was beneficial for partial nitrification, and it could be adopted for regulating the nitrite production.

Keywords: AHLs; Anammox; Denitrification; N-dodecanoyl homoserine lactone (C12-HSL); Nitrogen removal; Quorum sensing.

MeSH terms

4-Butyrolactone / pharmacology
Acyl-Butyrolactones*
Anaerobic Ammonia Oxidation
Homoserine / metabolism
Lactones
Nitrogen
Quorum Sensing*

Substances

Acyl-Butyrolactones
Homoserine
4-Butyrolactone
Nitrogen
Lactones