Electrochemical DNAzyme-based biosensors for disease diagnosis

Richa Pandey; Yang Lu; Erin M McConnell; Enas Osman; Alexander Scott; Jimmy Gu; Todd Hoare; Leyla Soleymani; Yingfu Li

doi:10.1016/j.bios.2022.114983

Electrochemical DNAzyme-based biosensors for disease diagnosis

Biosens Bioelectron. 2023 Mar 15:224:114983. doi: 10.1016/j.bios.2022.114983. Epub 2022 Dec 30.

Authors

Richa Pandey¹, Yang Lu², Erin M McConnell³, Enas Osman², Alexander Scott², Jimmy Gu³, Todd Hoare⁴, Leyla Soleymani⁵, Yingfu Li⁶

Affiliations

¹ Department of Engineering Physics, McMaster University, Hamilton, Ontario, L8S 4K1, Canada; Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, T2N 1N4, Canada. Electronic address: richa.pandey@ucalgary.ca.
² School of Biomedical Engineering, McMaster University, Hamilton, Ontario, L8S 4K1, Canada.
³ Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, L8S 4K1, Canada.
⁴ School of Biomedical Engineering, McMaster University, Hamilton, Ontario, L8S 4K1, Canada; Department of Chemical Engineering, McMaster University, Hamilton, Ontario, L8S 4K1, Canada.
⁵ Department of Engineering Physics, McMaster University, Hamilton, Ontario, L8S 4K1, Canada; School of Biomedical Engineering, McMaster University, Hamilton, Ontario, L8S 4K1, Canada; Michael G. DeGroot Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, L8S 4K1, Canada. Electronic address: soleyml@mcmaster.ca.
⁶ School of Biomedical Engineering, McMaster University, Hamilton, Ontario, L8S 4K1, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, L8S 4K1, Canada; Michael G. DeGroot Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, L8S 4K1, Canada. Electronic address: liying@mcmaster.ca.

PMID: 36640547
DOI: 10.1016/j.bios.2022.114983

Abstract

DNAzyme-based electrochemical biosensors provide exceptional analytical sensitivity and high target recognition specificity for disease diagnosis. This review provides a critical perspective on the fundamental and applied impact of incorporating DNAzymes in the field of electrochemical biosensing. Specifically, we highlight recent advances in creating DNAzyme-based electrochemical biosensors for diagnosing infectious diseases, cancer and regulatory diseases. We also develop an understanding of challenges around translating the research in the field of DNAzyme-based electrochemical biosensors from labs to clinics, followed by a discussion on different strategies that can be applied to enhance the performance of the currently existing technologies to create truly point-of-care electrochemical DNAzyme biosensors.

Keywords: DNAzyme; Electrochemical biosensor; Functional nucleic acid; Point of care diagnostics.

Publication types

Review

MeSH terms

Biosensing Techniques*
DNA, Catalytic*
Electrochemical Techniques
Point-of-Care Systems

Substances

DNA, Catalytic