Chitosan-based packaging films with an integrated antimicrobial peptide: Characterization, in vitro release and application to fresh pork preservation

Xiaofang Luo; Yafang Peng; Zuodong Qin; Wufei Tang; Gregory J Duns; Wubliker Dessie; Nongyue He; Yimin Tan

doi:10.1016/j.ijbiomac.2023.123209

Chitosan-based packaging films with an integrated antimicrobial peptide: Characterization, in vitro release and application to fresh pork preservation

Int J Biol Macromol. 2023 Mar 15:231:123209. doi: 10.1016/j.ijbiomac.2023.123209. Epub 2023 Jan 10.

Authors

Xiaofang Luo¹, Yafang Peng², Zuodong Qin³, Wufei Tang², Gregory J Duns¹, Wubliker Dessie², Nongyue He⁴, Yimin Tan⁵

Affiliations

¹ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China; Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, 425199 Yongzhou, China.
² Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, 425199 Yongzhou, China.
³ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China; Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, 425199 Yongzhou, China. Electronic address: qinzd@huse.edu.cn.
⁴ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.
⁵ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China. Electronic address: yimin.tan@hut.edu.cn.

PMID: 36639078
DOI: 10.1016/j.ijbiomac.2023.123209

Abstract

Chitosan (CS) films were developed incorporating peptide HX-12C. The films were studied to determine their microstructures, physical properties, release properties of peptide HX-12C and functional properties. The results indicated that there may be hydrogen bonding interactions between CS and peptide HX-12C, thereby creating a homogeneous internal microstructure and lower crystallinity (10.8-12.8 %). Compared with CS film, CS-HX-12C films displayed lower light transmission, MC (20.8-19.9 %), WVP (8.82-8.59 × 10^-11·g·m^-1·s^-1·Pa^-1), OTR (0.015-0.037 cc/(m².day)) and higher WS (15.7-32.4 %) values. Moreover, controlled-release experiments showed that pH, ionic strength and temperature could all significantly affect the release of peptide HX-12C from the films. Finally, the increase of pH value and TVC and lipid oxidation of fresh pork were delayed due to the treatment with CS-2%HX-12C film. However, incorporating peptide HX-12C into CS films did not improve the mechanical properties of the films and their effects against protein oxidation. Our results suggest that the CS-based antimicrobial packaging films integrated with peptide HX-12C exhibit the potential for fresh pork preservation.

Keywords: Antimicrobial peptide HX-12C; Chitosan-based packaging film; Fresh pork preservation.

MeSH terms

Animals
Antimicrobial Peptides
Chitosan* / chemistry
Food Packaging / methods
Pork Meat*
Red Meat*
Swine

Substances

Chitosan
Antimicrobial Peptides