Transcriptome analysis of Macrobrachium rosenbergii hemocytes reveals in-depth insights into the immune response to Vibrio parahaemolyticus infection

Fanbin Zhan; Yanan Li; Fei Shi; Zhijie Lu; Minxuan Yang; Qingqing Li; Li Lin; Zhendong Qin

doi:10.1016/j.fsi.2023.108533

Transcriptome analysis of Macrobrachium rosenbergii hemocytes reveals in-depth insights into the immune response to Vibrio parahaemolyticus infection

Fish Shellfish Immunol. 2023 Feb:133:108533. doi: 10.1016/j.fsi.2023.108533. Epub 2023 Jan 11.

Authors

Fanbin Zhan¹, Yanan Li¹, Fei Shi¹, Zhijie Lu¹, Minxuan Yang¹, Qingqing Li¹, Li Lin², Zhendong Qin³

Affiliations

¹ Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China.
² Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China. Electronic address: linli@zhku.edu.cn.
³ Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China. Electronic address: qinzhendongsc@163.com.

PMID: 36639067
DOI: 10.1016/j.fsi.2023.108533

Abstract

Macrobrachium rosenbergii as one of the common freshwater prawn species in Southeast Asia, which breeding industry is seriously threatened by vibriosis and causes high mortality. In this study, the RNA-seq was employed for assessing the M. rosenbergii hemocytes transcriptomes following Vibrio parahaemolyticus challenge. After challenge for 6 h (h), there were overall 1849 DEGs or differentially expressed genes, including 1542 up-regulated and 307 down-regulated genes, and there was a total of 1048 DEGs, including 510 up-regulated genes and 538 down-regulated genes, after challenge for 12 h. Mitogen-activated protein kinase (MAPK) immune-related pathways, Toll, immune deficiency (IMD), and Janus kinase (JAK)/signal transducer and activator of transcription (STAT) were among the immune pathways where a lot of the DEGs were connected. The expression patterns of 18 chosen immune-related genes were examined utilizing qRT-PCR or quantitative real-time polymerase chain reaction, which revealed that the V. parahaemolyticus infection activated the M. rosenbergii's immune response. Permutational multivariate analysis of variance (PERMANOVA) showed that V. parahaemolyticus infection modulated immune regulation and apoptosis pathways. The gathered information provided new insight into M. rosenbergii's immunity and suggested a novel approach to fight against bacterial infection.

Keywords: Hemocytes; Immune genes; Macrobrachium rosenbergii; Vibrio parahaemolyticus.

MeSH terms

Animals
Gene Expression Profiling
Hemocytes
Immunity
Immunity, Innate / genetics
Palaemonidae*
Transcriptome
Vibrio Infections* / metabolism
Vibrio parahaemolyticus* / physiology