Improvement of transformation efficiency in hypermucoviscous Klebsiella pneumoniae using citric acid

Suyeon Park; Kwan Soo Ko

doi:10.1016/j.mimet.2023.106673

Improvement of transformation efficiency in hypermucoviscous Klebsiella pneumoniae using citric acid

J Microbiol Methods. 2023 Feb:205:106673. doi: 10.1016/j.mimet.2023.106673. Epub 2023 Jan 10.

Authors

Suyeon Park¹, Kwan Soo Ko²

Affiliations

¹ Department of Microbiology, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea.
² Department of Microbiology, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea. Electronic address: ksko@skku.edu.

PMID: 36638870
DOI: 10.1016/j.mimet.2023.106673

Abstract

Transformation efficiency is very low in hypermucoviscous Kllebsiella pneumoniae owing to high amount of capsular polysaccharide. Here, we propose a method to improve the transformation in hypermucoviscous K. pneumoniae using citric acid. An increased number of recovered transformants was observed in the strains cultured in the presence of citric acid.

Keywords: Capsular polysaccharide (CPS); Citric acid; Hypermucoviscosity; Klebsiella pneumoniae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Citric Acid
Humans
Klebsiella Infections*
Klebsiella pneumoniae* / genetics

Substances

Citric Acid