A microfluidic immunosensor based on magnetic separation for rapid detection of okadaic acid in marine shellfish

Yuxiang Ji; Gaozhe Cai; Cheng Liang; Zehang Gao; Weimin Lin; Zizhen Ming; Shilun Feng; Hongwei Zhao

doi:10.1016/j.aca.2022.340737

A microfluidic immunosensor based on magnetic separation for rapid detection of okadaic acid in marine shellfish

Anal Chim Acta. 2023 Jan 25:1239:340737. doi: 10.1016/j.aca.2022.340737. Epub 2022 Dec 21.

Authors

Yuxiang Ji¹, Gaozhe Cai², Cheng Liang³, Zehang Gao⁴, Weimin Lin⁵, Zizhen Ming⁶, Shilun Feng⁷, Hongwei Zhao⁸

Affiliations

¹ State Key Laboratory of Marine Resources Utilization in South China Sea and Center for Eco-Environment Restoration of Hainan Province, Hainan University, Haikou, 570228, China; Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical University, Haikou, 571199, China.
² State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
³ State Key Laboratory of Marine Resources Utilization in South China Sea and Center for Eco-Environment Restoration of Hainan Province, Hainan University, Haikou, 570228, China.
⁴ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Department of Clinical Laboratory, Third Affiliated Hospital of Guangzhou Medical University, Guangdong, 510150, China.
⁵ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China.
⁶ Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
⁷ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China. Electronic address: shilun.feng@mail.sim.ac.cn.
⁸ State Key Laboratory of Marine Resources Utilization in South China Sea and Center for Eco-Environment Restoration of Hainan Province, Hainan University, Haikou, 570228, China. Electronic address: hwzhao@hainanu.edu.cn.

PMID: 36628732
DOI: 10.1016/j.aca.2022.340737

Abstract

Okadaic acid (OA) is a marine biotoxin that accumulates in seafood and can cause diarrheic shellfish poisoning if consumed. Accordingly, many countries have established regulatory limits for the content of OA in shellfish. At present, methods used for the detection of marine toxins are time-consuming and labor-intensive. In order to realize rapid, simple, and accurate detection of OA, we developed a novel microfluidic immunosensor based on magnetic beads modified with a highly specific and sensitive monoclonal antibody (mAb) against OA that is used in conjunction with smartphone imaging to realize the rapid detection of OA in shellfish. The method achieves on-site detection results within 1 h with an IC₅₀ value of 3.30 ng/mL for OA and a limit of detection (LOD) of 0.49 ng/mL. In addition, the analysis of real samples showed that the recoveries for spiked shellfish samples ranged from 84.91% to 95.18%, and the results were confirmed by indirect competitive enzyme-linked immunosorbent assay (icELISA), indicating that the method has good accuracy and precision. Furthermore, the results are reported in a specially designed smartphone app. The microfluidic immunosensor has the advantages of simple operation, rapid detection, and high sensitivity, providing a reliable technical solution for detecting OA residues in shellfish.

Keywords: Marine biotoxins; Microfluidic immunosensor; Okadaic acid; Shellfish; Smart phone imaging.

MeSH terms

Biosensing Techniques*
Immunoassay / methods
Magnetic Phenomena
Microfluidics
Okadaic Acid / analysis
Seafood / analysis
Shellfish / analysis

Substances

Okadaic Acid