Triisobutyl phosphate biodegradation by enriched activated sludge consortia: Degradation mechanism and bioaugmentation potential

Abstract

This study investigated the ability of activated sludge (AS) to biodegrade triisobutyl phosphate (TiBP) after acclimation in an AS bioreactor by adding 50 mg/L TiBP. The bioreactor significantly increased the biotransformation rate of TiBP (2.15-12.7 d^-1) over two months of acclimation. Seven transformation products (TPs) of TiBP were identified by high-resolution mass spectrometry, and hydrolysis, hydroxylation and dehydrogenation were the major biodegradation pathways of TiBP. TiBP degradation solutions at 0, 3, 7, and 10 h showed significantly toxic effects on zebrafish embryos, while the toxicity of TiBP degradation solutions at 24 h significantly decreased. Pseudomonas was inferred to be a specific bacterial population in the TiBP metabolic microbial consortium (TMMC) that degrades TiBP (p < 0.001). When TMMC (0.5, 1, and 2 g_ss/L) was introduced into AS, the TiBP biotransformation rates (1.97, 2.05, and 2.26 d^-1 at 1.0 mg/L TiBP, and 0.09, 0.11, and 0.83 d^-1 at 30.0 mg/L TiBP) were significantly enhanced compared to the control (0.31 and 0.07 d^-1) without TMMC inoculation. In general, this study provides new insights into the key species populations that accelerate TiBP degradation and promote the development of TiBP reduction biotechnology in WWTPs.

Keywords: Bioaugmentation potential; Biotransformation pathway; Microbial consortium; Toxic; Triisobutyl phosphate.