A novel paper-based lysis strip for SARS-CoV-2 RNA detection at low resource settings

Songhyun Lee; SeJin Kim; Sanghyo Kim

doi:10.1016/j.ab.2023.115037

A novel paper-based lysis strip for SARS-CoV-2 RNA detection at low resource settings

Anal Biochem. 2023 Mar 1:664:115037. doi: 10.1016/j.ab.2023.115037. Epub 2023 Jan 6.

Authors

Songhyun Lee¹, SeJin Kim¹, Sanghyo Kim²

Affiliations

¹ Department of Bionanotechnology, Gachon University, Seongnam, 13120, Republic of Korea.
² Department of Bionanotechnology, Gachon University, Seongnam, 13120, Republic of Korea. Electronic address: samkim@gachon.ac.kr.

Abstract

Infectious respiratory diseases such as COVID-19 are serious and global concerns from the past to the present. To isolate the spread of infectious diseases even in the absence of a health system, a simple, inexpensive, reliable, sensitive, and selective molecular diagnosis platform for Point of Care Test (POCT) is required. Especially, the nucleic acid extraction step is difficult to perform out of laboratory. Here, we propose a paper-based lysis (PBL) strip for nucleic acid extraction, especially in low-resource settings (LRS). PBL strips are suitable for isolating RNA from viruses with biological interference and inhibitors. We optimized the buffer compositions and membranes of the strip. A simple preparation method using a PBL strip could obtain an eluent for downstream inspection within 20 min. Overall, 10⁴ copies/swaps were detected for 20 min for amplification in combination with Reverse Transcription Loop-Mediated Amplification (RT-LAMP).

Keywords: ARS-CoV-2; Lysis; Paper-based; Point of care test; RNA extraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19 Testing
COVID-19* / diagnosis
Humans
Nucleic Acid Amplification Techniques / methods
Nucleic Acids*
RNA, Viral / genetics
SARS-CoV-2 / genetics
Sensitivity and Specificity

Substances

RNA, Viral
Nucleic Acids