Da-Cheng-Qi decoction improves severe acute pancreatitis-associated acute lung injury by interfering with intestinal lymphatic pathway and reducing HMGB1-induced inflammatory response in rats

Xiaowen Liu; Lin Yuan; Yishuang Tang; Yue Wu; Jing Kong; Bingduo Zhou; Xiaosu Wang; Min Lin; Yading Li; Gaofan Xu; Yi Wang; Tingting Xu; Cong He; Shengquan Fang; Shengliang Zhu

doi:10.1080/13880209.2022.2160768

Da-Cheng-Qi decoction improves severe acute pancreatitis-associated acute lung injury by interfering with intestinal lymphatic pathway and reducing HMGB1-induced inflammatory response in rats

Pharm Biol. 2023 Dec;61(1):144-154. doi: 10.1080/13880209.2022.2160768.

Authors

Xiaowen Liu¹, Lin Yuan², Yishuang Tang¹, Yue Wu¹, Jing Kong¹, Bingduo Zhou¹, Xiaosu Wang¹, Min Lin², Yading Li¹, Gaofan Xu¹, Yi Wang¹, Tingting Xu¹, Cong He¹, Shengquan Fang¹, Shengliang Zhu¹

Affiliations

¹ Department of Gastroenterology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
² Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Abstract

Context: Da-Cheng-Qi Decoction (DCQD) has a significant effect on Severe Acute Pancreatitis-Associated Acute Lung Injury (SAP-ALI).

Objective: To explore the mechanism of DCQD in the treatment of SAP-ALI based on intestinal barrier function and intestinal lymphatic pathway.

Materials and methods: Forty-five Sprague-Dawley rats were divided into three groups: sham operation, model, and DCQD. The SAP model was induced by a retrograde infusion of 5.0% sodium taurocholate solution (1 mg/kg) at a constant rate of 12 mL/h using an infusion pump into the bile-pancreatic duct. Sham operation and model group were given 0.9% normal saline, while DCQD group was given DCQD (5.99 g/kg/d) by gavage 1 h before operation and 1, 11 and 23 h after operation. The levels of HMGB1, RAGE, TNF-α, IL-6, ICAM-1, d-LA, DAO in blood and MPO in lung were detected using ELISA. The expression of HMGB1, RAGE, NF-κB p65 in mesenteric lymph nodes and lung were determined.

Results: Compared with SAP group, DCQD significantly reduced the histopathological scoring of pancreatic tissue (SAP, 2.80 ± 0.42; DCQD, 2.58 ± 0.52), intestine (SAP, 3.30 ± 0.68; DCQD, 2.50 ± 0.80) and lung (SAP, 3.30 ± 0.68; DCQD, 2.42 ± 0.52). DCQD reduced serum HMGB1 level (SAP, 134.09 ± 19.79; DCQD, 88.05 ± 9.19), RAGE level (SAP, 5.05 ± 1.44; DCQD, 2.13 ± 0.54). WB and RT-PCR showed HMGB1-RAGE pathway was inhibited by DCQD (p < 0.01).

Discussion and conclusions: DCQD improves SAP-ALI in rats by interfering with intestinal lymphatic pathway and reducing HMGB1-induced inflammatory response.

Keywords: Intestinal barrier function; NF-κB p65; traditional Chinese medicine.

MeSH terms

Acute Disease
Acute Lung Injury* / drug therapy
Acute Lung Injury* / etiology
Animals
HMGB1 Protein*
Intestines
Pancreatitis* / drug therapy
Rats
Rats, Sprague-Dawley

Substances

Da-Cheng-Qi
HMGB1 Protein

Grants and funding

This work was supported by the National Natural Science Foundation of China (Nos.81302929, 81973809), Shanghai High level Talents Guidance Plan for Traditional Chinese Medicine (No.ZY(2021-2023)-0403), Shanghai Municipal Health Commission-Traditional Chinese Medicine Innovation Team (No. 2022CX002), Shanghai Municipal Health Commission-Zhu Shengliang's Academic Experience Research Studio Project of Shanghai Famous Traditional Chinese Medicine (No. SHGZS-202203).