Soil Acidobacterial community composition changes sensitively with wetland degradation in northeastern of China

Xin Sui; Beat Frey; Libin Yang; Yingnan Liu; Rongtao Zhang; Hongwei Ni; Mai-He Li

doi:10.3389/fmicb.2022.1052161

Soil Acidobacterial community composition changes sensitively with wetland degradation in northeastern of China

Front Microbiol. 2022 Dec 23:13:1052161. doi: 10.3389/fmicb.2022.1052161. eCollection 2022.

Authors

Xin Sui^{1

2

3

4}, Beat Frey³, Libin Yang⁴, Yingnan Liu⁴, Rongtao Zhang⁴, Hongwei Ni⁵, Mai-He Li^{3

6

7}

Affiliations

¹ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, China.
² Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin, China.
³ Snow and Landscape Research WSLSwiss Federal Institute for Forest, , Birmensdorf, Switzerland.
⁴ Institute of Nature and Ecology, Heilongjiang Academy of Sciences, Harbin, China.
⁵ Heilongjiang Academy of Forestry, Harbin, China.
⁶ Key Laboratory of Geographical Processes and Ecological Security in Changbai Mountains, Ministry of Education, School of Geographical Sciences, Northeast Normal University, Changchun, China.
⁷ School of Life Science, Hebei University, Baoding, China.

Abstract

Acidobacteria are a major component of the soil bacteria and are conducted for many soil functions, and the soil Acidobacterial structure and diversity are affected by climate changes and human activities. However, soil Acidobacterial structure and diversity in wetland ecosystems are still limited recognized. The current study aimed to study the Acidobacterial community and diversity in relation to soil environmental factors along a typical degradation series from primitive wetland to forest in a representative fresh wetland in northeastern China. In this research, we assessed the soil Acidobacterial community composition, using Illumina MiSeq sequencing along a typical degradation series from primitive wetland to forest in a representative fresh wetland in northeastern China. The soil physico chemical properties changed significantly among the eight degrade stages (p < 0.05). The α diversity index (Shannon and Chao1 index) of soil Acidobacteria changed significantly between different degradation stages (p < 0.05). Principal Coordinates Analysis (PCoA) revealed that the soil acidobacteiral communities obviously separated into wetland group and forest group. The most abundant subgroups of Acidobacteria accounted for 31% (Gp1), 5% (Gp2), 12% (Gp3), 2% (Gp4), 5% (Gp6), and 2% (Gp7) in soils within eight successional series. The compositions of soil Acidobacteria in wetland stages were significantly affected by soil moisture content, soil total nitrogen and available nitrogen contents, while those in forest stages were significantly driven by soil pH, soil organic carbon, total nitrogen, available phosphorus and soil moisture content. Our results indicated that the soil Acidobacterial community was mainly structured by soil physico chemical parameters, and wetland degradation towards forests will greatly influence the soil Acidobacterial structure and thus the wetland functions.

Keywords: Sanjiang plain; community structure; forest; high-throughput sequencing; soil bacterial diversity; β diversity.