Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest

Junwen Cheng; Yanbin Wang; Jiling Song; Yu Liu; Weiwei Ji; Liang He; Hailong Wei; Chuanjiu Hu; Yihan Jiang; Yiqi Xing; Xubo Huang; Hongmei Ding; Qinghai He

doi:10.3389/fnut.2022.1058131

Characterization, immunostimulatory and antitumor activities of a β-galactoglucofurannan from cultivated Sanghuangporus vaninii under forest

Front Nutr. 2022 Dec 22:9:1058131. doi: 10.3389/fnut.2022.1058131. eCollection 2022.

Authors

Junwen Cheng¹, Yanbin Wang¹, Jiling Song², Yu Liu³, Weiwei Ji⁴, Liang He¹, Hailong Wei¹, Chuanjiu Hu¹, Yihan Jiang^{1

5}, Yiqi Xing^{1

5}, Xubo Huang¹, Hongmei Ding⁶, Qinghai He¹

Affiliations

¹ Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou, China.
² Institute of Horticulture, Hangzhou Academy of Agricultural Sciences, Hangzhou, China.
³ Institute of Biochemistry, College of Life Sciences, Institute of Biochemistry, Zhejiang University, Hangzhou, China.
⁴ Huzhou Liangxi Forest Park Management Office, Huzhou, China.
⁵ School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, China.
⁶ Center of Forecasting and Analysis, Zhejiang Chinese Medical University, Hangzhou, China.

Abstract

A biomacromolecule, named as β-galactoglucofurannan (SVPS2), was isolated from the cultivated parts of Sanghuangporus vaninii under the forest. Its primary and advanced structure was analyzed by a series of techniques including GC-MS, methylation, NMR, MALS as well as AFM. The results indicated that SVPS2 was a kind of 1, 5-linked β-Glucofurannan consisting of β-glucose, β-galactose and α-fucose with 23.4 KDa. It exhibited a single-stranded chain with an average height of 0.72 nm in saline solution. The immunostimulation test indicated SVPS2 could facilitate the initiation of the immune reaction and promote the secretion of cytokines in vitro. Moreover, SVPS2 could mediate the apoptosis of HT-29 cells by blocking them in S phase. Western blot assay revealed an upregulation of Bax, Cytochrome c and cleaved caspase-3 by SVPS2, accompanied by a downregulation of Bcl-2. These results collectively demonstrate that antitumor mechanism of SVPS2 may be associated with enhancing immune response and inducing apoptosis of tumor cells in vitro. Therefore, SVPS2 might be utilized as a promising therapeutic agent against colon cancer and functional food with immunomodulatory activity.

Keywords: Sanghuangporus vaninii; antitumor activity; conformation; polysaccharide; structure.