Automated high-content imaging in iPSC-derived neuronal progenitors

Apostolos Papandreou; Christin Luft; Serena Barral; Janos Kriston-Vizi; Manju A Kurian; Robin Ketteler

doi:10.1016/j.slasd.2022.12.002

Automated high-content imaging in iPSC-derived neuronal progenitors

SLAS Discov. 2023 Mar;28(2):42-51. doi: 10.1016/j.slasd.2022.12.002. Epub 2023 Jan 5.

Authors

Apostolos Papandreou¹, Christin Luft², Serena Barral³, Janos Kriston-Vizi², Manju A Kurian³, Robin Ketteler²

Affiliations

¹ University College London MRC Laboratory for Molecular Cell Biology, London, UK; Developmental Neurosciences, Zayed Centre for Research into Rare Disease in Children, University College London Great Ormond Street Institute of Child Health, London, UK.
² University College London MRC Laboratory for Molecular Cell Biology, London, UK.
³ Developmental Neurosciences, Zayed Centre for Research into Rare Disease in Children, University College London Great Ormond Street Institute of Child Health, London, UK.

Abstract

Induced pluripotent stem cells (iPSCs) have great potential as physiological disease models for human disorders where access to primary cells is difficult, such as neurons. In recent years, many protocols have been developed for the generation of iPSCs and the differentiation into specialised cell subtypes of interest. More recently, these models have been modified to allow large-scale phenotyping and high-content screening of small molecule compounds in iPSC-derived neuronal cells. Here, we describe the automated seeding of day 11 ventral midbrain progenitor cells into 96-well plates, administration of compounds, automated staining for immunofluorescence, the acquisition of images on a high-content screening platform and workflows for image analysis.

Keywords: High-content screening; Image analysis; Immunofluorescence; Lab automation; Multi-well plates; Neurons.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / physiology
Cells, Cultured
Humans
Image Processing, Computer-Assisted
Induced Pluripotent Stem Cells*
Neurons

Abstract

Publication types

MeSH terms

Grants and funding