N-doped carbon nanospheres as selective fluorescent probes for mercury detection in contaminated aqueous media: chemistry, fluorescence probing, cell line patterning, and liver tissue interaction

Abstract

A precise nano-scale biosensor was developed here to detect Hg²⁺ in aqueous media. Nitrogen-doped carbon nanospheres (NCS) created from the pyrolysis of melamine-formaldehyde resin were characterized by FESEM, XRD, Raman spectra, EDS, PL, UV-vis spectra, and N₂ adsorption-desorption, and were used as a highly selective and sensitive probe for detecting Hg²⁺ in aqueous media. The sensitivity of NCS to Hg²⁺ was evaluated by photoluminescence intensity fluctuations under fluorescence emission in the vicinity of 390 nm with a λ_exc of 350 nm. The fluorescence intensity of the NCS probe weakened in the presence of Hg²⁺ owing to the effective fluorescence quenching by that, which is not corresponding to the special covalent liking between the ligand and the metal. The effects of the fluorescence nanoprobe concentration, pH, and sensing time were monitored to acquire the best conditions for determining Hg²⁺. Surprisingly, NCS revealed excellent selectivity and sensitivity towards Hg²⁺ in the samples containing Co²⁺, Na⁺, K⁺, Fe²⁺, Mn²⁺, Al³⁺, Pb²⁺, Ni²⁺, Ca²⁺, Cu²⁺, Mg²⁺, Cd²⁺, Cr³⁺, Li⁺, Cs⁺, and Ba²⁺. The fluorescence response was linearly proportional to Hg²⁺ concentration in 0.013-0.046 µM with a limit of detection of 9.58 nM. The in vitro and in vivo toxicological analyses confirmed the completely safe and biocompatible features of NCS, which provides promise for use for water, fruit, vegetable, and/or other forms of natural-connected materials exposed to Hg²⁺, with no significant toxicity noticed toward different cells/organs/tissues.

Keywords: Fluorescence probe; Hazardous materials; Hg2+; Melamine formaldehyde resin; Nitrogen-doped carbon spheres; Ultrasonic spray pyrolysis.