Photothermal and selective microbial inactivation behaviors of gluconamide-coated IR780 nanoparticles

Tiantian Xu; Weiqi Liu; Zhi Li; Bo Wang; Bimal Chitrakar; Wenguang Xiong; Yahong Liu; Zhenling Zeng

doi:10.1016/j.colsurfb.2023.113126

Photothermal and selective microbial inactivation behaviors of gluconamide-coated IR780 nanoparticles

Colloids Surf B Biointerfaces. 2023 Feb:222:113126. doi: 10.1016/j.colsurfb.2023.113126. Epub 2023 Jan 2.

Authors

Tiantian Xu¹, Weiqi Liu¹, Zhi Li¹, Bo Wang², Bimal Chitrakar³, Wenguang Xiong⁴, Yahong Liu¹, Zhenling Zeng⁵

Affiliations

¹ Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, China.
² School of Food and Biological Engineering, Jiangsu University, 212013 Zhenjiang, Jiangsu, China.
³ College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
⁴ Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, China. Electronic address: xiongwg@scau.edu.cn.
⁵ Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; National Reference Laboratory of Veterinary Drug Residues, South China Agricultural University, Guangzhou 510642, China. Electronic address: zlzeng@scau.edu.cn.

PMID: 36608368
DOI: 10.1016/j.colsurfb.2023.113126

Abstract

Photothermal therapy (PTT) is a promising alternative treatment for bacterial infection. In this study, a photothermal nanoparticle was prepared by encapsulating IR780 into N-octyl-D-gluconamide (GA). The photothermal nanoparticle (IR780-GA NP) was evenly suspended in water with an average particle size of 42.2 nm. After exposure to near-infrared light, the temperature of the IR780-GA NP suspension was increased by around 15 °C within 5 min. This leads to an obvious microbial inactivation effect when it is adsorbed to methicillin-resistant Staphylococcus aureus (MRSA, 2 orders of magnitude reduction of CFU concentration) and Escherichia coli (1.5 orders of magnitude reduction of CFU concentration). Interestingly, Salmonella typhimurium survived after the same treatment. Different strains also showed variations. The hemolysis test showed that IR780-GA NPs had good blood compatibility. In vivo experiments collaborated with the in vitro findings. The IR780-GA NP-triggered photothermal effects killed 63-100% of bacteria in the wound site of mice depending on the IR780-GA NP concentration. Overall, this study provided the fundamental basis of IR780-GA NPs in four aspects: fabrication, photothermal characterization, selective adsorption, and microbial inactivation (in vitro and in vivo). The findings of this study provide a practical approach for the development of mild photothermal therapy which targets specific bacterial strains and treats MRSA infection effectively.

Keywords: Adsorption; IR780; Microbial inactivation; N-octyl-D-gluconamide; Photothermal materials.

MeSH terms

Animals
Methicillin-Resistant Staphylococcus aureus*
Mice
Microbial Viability
Nanoparticles*
Photochemotherapy*

Substances

gluconamide