A gain-of-function mutation in head involution defective , Wrinkled, causes precocious cell death of wing epidermal cells in Drosophila

Takumi Ohta; Teiichi Tanimura; Ken-Ichi Kimura

doi:10.17912/micropub.biology.000703

A gain-of-function mutation in head involution defective , Wrinkled, causes precocious cell death of wing epidermal cells in Drosophila

MicroPubl Biol. 2022 Dec 20:2022:10.17912/micropub.biology.000703. doi: 10.17912/micropub.biology.000703. eCollection 2022.

Authors

Takumi Ohta^{1

2}, Teiichi Tanimura^{2

3}, Ken-Ichi Kimura¹

Affiliations

¹ Laboratory of Biology, Hokkaido University of Education, Sapporo Campus, Sapporo, Japan.
² Department of Biology, Graduate School of Science, Kyushu University, Fukuoka, Japan.
³ Present address: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan.

Abstract

In Drosophila , wing epidermal cells undergo programmed cell death as the last step of metamorphosis. The aim of this study was to evaluate the role of hid , particularly the Wrinkled mutation ( hid ^W ), an allele of hid , in the cell death. The wing epithelial cell death is suppressed by loss-of-function mutation of hid , indicating that the death is governed by a cascade involving hid . Examination of the cell death in hid ^W showed that precocious death started at G stage, 3 h before eclosion. Thus, mutated-HID in the hid ^W mutant was activated at G stage, supporting the gain-of-function effect of hid ^W mutation.