The enhancement effect of low-dose dietary lipopolysaccharide on the growth and immunity of Litopenaeus vannamei, and transcriptome analysis

Zhao Yan; Lulu Zhu; Cuihong Hou; Yudong Zheng; Hui Guo; Lili Shi; Beiping Tan; Shuang Zhang

doi:10.1016/j.fsi.2023.108517

The enhancement effect of low-dose dietary lipopolysaccharide on the growth and immunity of Litopenaeus vannamei, and transcriptome analysis

Fish Shellfish Immunol. 2023 Feb:133:108517. doi: 10.1016/j.fsi.2023.108517. Epub 2023 Jan 2.

Authors

Zhao Yan¹, Lulu Zhu¹, Cuihong Hou¹, Yudong Zheng¹, Hui Guo¹, Lili Shi¹, Beiping Tan², Shuang Zhang³

Affiliations

¹ College of Fisheries, Guangdong Ocean University, Zhanjiang, China.
² College of Fisheries, Guangdong Ocean University, Zhanjiang, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, China.
³ College of Fisheries, Guangdong Ocean University, Zhanjiang, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, China. Electronic address: zshuang@gdou.edu.cn.

PMID: 36603789
DOI: 10.1016/j.fsi.2023.108517

Abstract

In this study, the effects of dietary lipopolysaccharide (LPS) on Litopenaeus vannamei were investigated to determine whether LPS could play a role as a potential immunostimulant in shrimp. L. vannamei with an initial body weight of 0.30 ± 0.02 g were fed a diet containing LPS at doses of 0, 0.2, 1, 5, 25 or 125 mg kg^-1 for eight weeks (groups LPS0, LPS0.2, LPS1, LPS5, LPS25 and LPS125, respectively). After eight weeks of feeding, the growth performance, immunity and transcriptome response of L. vannamei were analysed. Only dietary LPS at 0.2 and 1 mg kg^-1 resulted in a significant increase in the growth of L. vannamei (P < 0.05). According to the weight gain rate (WGR) and specific growth rate (SGR), the optimum dietary LPS level was 2.462 and 2.455 mg kg^-1, respectively. When compared with the control group, the survival rate (SR) of L. vannamei in the LPS0.2 group was significantly increased after white spot syndrome virus (WSSV) infection and the SR of L. vannamei in the LPS1 group was significantly increased after Vibrio parahaemolyticus infection (both P < 0.05). Compared with the LPS0 group, immune enzyme activity in the serum of L. vannamei could be significantly increased and the content of maleic dialdehyde (MDA) significantly decreased by dietary LPS. Transcriptome analysis of the haemocytes of L. vannamei identified 399 up-regulated differentially expressed genes (DEGs) and 5000 down-regulated DEGs in the LPS0.2 compared to the control group. Most of the DEGs were significantly enriched in the following pathways: phosphatidylinositol signalling, Wnt signalling, Jak-STAT signalling and inositol phosphate metabolism. In conclusion, this study revealed that diets supplemented with low-dose LPS had positive effects on the growth and immunity of L. vannamei.

Keywords: Immune enhancement; LPS; Litopenaeus vannamei; Transcriptome analysis.

MeSH terms

Animal Feed / analysis
Animals
Diet / veterinary
Gene Expression Profiling
Immunity, Innate / genetics
Lipopolysaccharides / pharmacology
Penaeidae*
White spot syndrome virus 1* / genetics

Substances

Lipopolysaccharides