Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models

Bárbara Jonson Bartikoski; Larisse Longo; Valessa Emanoele Gabriel de Souza; Fernando Salvati; Carolina Uribe-Cruz; Guido Lenz; Ricardo Machado Xavier; Mário Reis Álvares-da-Silva; Fernanda Visioli; Eduardo Cremonese Filippi-Chiela

doi:10.1016/j.xpro.2022.101855

Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models

STAR Protoc. 2022 Dec 16;3(4):101855. doi: 10.1016/j.xpro.2022.101855. Epub 2022 Nov 16.

Affiliations

¹ Autoimmune Diseases Laboratory, Rheumatology Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, Porto Alegre, Rio Grande do Sul 90035-903, Brazil; Graduate Program in Medical Sciences, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2400, Porto Alegre, Rio Grande do Sul 90035-003, Brazil.
² Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2400, Porto Alegre, Rio Grande do Sul 90035-003, Brazil; Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, Porto Alegre, Rio Grande do Sul 90035-903, Brazil.
³ Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, Porto Alegre, Rio Grande do Sul 90035-903, Brazil.
⁴ School of Medicine, Instituto Meridional de Educação - IMED, Senador Pinheiro, 304, Passo Fundo, Rio Grande do Sul, Brazil.
⁵ Department of Biophysics and Biotechnology Center, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90035-003, Brazil.
⁶ Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2400, Porto Alegre, Rio Grande do Sul 90035-003, Brazil; Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, Porto Alegre, Rio Grande do Sul 90035-903, Brazil; Division of Gastroenterology, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, Porto Alegre, Rio Grande do Sul 90035-903, Brazil.
⁷ Graduate Program Science in Dentistry, School of Dentistry, UFRGS, Porto Alegre, Rio Grande do Sul 90035-003, Brazil; Experimental Research Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, Porto Alegre, Rio Grande do Sul 90035-903, Brazil.
⁸ Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2400, Porto Alegre, Rio Grande do Sul 90035-003, Brazil; Experimental Research Service, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos, 2350, Porto Alegre, Rio Grande do Sul 90035-903, Brazil; Department of Morphological Sciences, Universidade Federal do Rio Grande do Sul, Sarmento Leite 500, Porto Alegre, Rio Grande do Sul 90035-903, Brazil. Electronic address: eduardochiela@gmail.com.

Abstract

The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers' subpopulations, aiming to characterize physiopathological conditions related to muscle tissue. The main limitation of MusMA is the need for high-quality tissue slides and images and control samples to set up the analyses.

Keywords: Developmental biology; Microscopy; Tissue Engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Mice
Muscle Fibers, Skeletal*