Heterotrophic nitrification-aerobic denitrification performance of a novel strain, Pseudomonas sp. B-1, isolated from membrane aerated biofilm reactor

Abstract

A heterotrophic nitrification-aerobic denitrification (HN-AD) strain isolated from membrane aerated biofilm reactor (MABR) was identified as Pseudomonas sp. B-1, which could effectively utilize multiple nitrogen sources and preferentially consume NH₄-N. The maximum degradation efficiencies of NO₃-N, NO₂-N and NH₄-N were 98.04%, 94.84% and 95.74%, respectively. The optimal incubation time, shaking speed, carbon source, pH, temperature and C/N ratio were 60 h, 180 rpm, sodium succinate, 8, 30 °C and 25, respectively. The strain preferred salinity of 1.5% and resisted heavy metals in the order of Mn²⁺ > Co²⁺ > Zn²⁺ > Cu²⁺. It can be preliminarily speculated from the results of enzyme assay that the strain removed nitrogen via full nitrification-denitrification pathway. The addition of strain into the conventional MABR significantly intensified the HN-AD performance of the reactor. The relative abundance of the functional bacteria including Flavobacterium, Pseudomonas, Paracoccus, Azoarcus and Thauera was obviously increased after the bioaugmentation. Besides, the expression of the HN-AD related genes in the biofilm was also strengthened. Thus, strain B-1 had great application potential in nitrogen removal process.

Keywords: Bioaugmentation; Heterotrophic nitrification-aerobic denitrification; Membrane aerated biofilm reactor; Nitrogen removal characteristic; Nitrogen transformation pathway; Pseudomonas anguilliseptica.