PDGF-β and IL-18 Expressions on Carcinoma Cervical by Rhodomyrtus tomentosa

Abstract

&lt;b&gt;Background and Objective:&lt;/b&gt; Cervical cancer, along with lung and breast cancer, is one of Indonesia's most aggressive gynaecological diseases. &lt;i&gt;Rhodomyrtus tomentosa&lt;/i&gt; has antioxidant and antiproliferative properties that could be developed into herbal medicines for molecular therapy. The IL-18 and PDGF-β are tumour-promoting agent proteins that may be therapeutic targets for a variety of cancers that were investigated in this study. &lt;b&gt;Materials and Methods:&lt;/b&gt; Rats were classified into five groups: Group C- is the control group, Group C+ is the cancer model group and Group RHO200 is the &lt;i&gt;Rhodomyrtus tomentosa&lt;/i&gt; 100 mg&lt;sup&gt;1&lt;/sup&gt; b.wt., rat group, Group RHO400 is the &lt;i&gt;Rhodomyrtus tomentosa&lt;/i&gt; 200 mg&lt;sup&gt;1&lt;/sup&gt; b.wt., rat group and Group RHO400 is the &lt;i&gt;Rhodomyrtus tomentosa&lt;/i&gt; 400 mg&lt;sup&gt;1&lt;/sup&gt; b.wt., rat group. The rats were dissected 30 days after receiving &lt;i&gt;Rhodomyrtus tomentosa&lt;/i&gt;. Immunohistochemistry is used to stain cervical tissues. &lt;b&gt;Results:&lt;/b&gt; The expression of IL-18 and PDGF-β was significantly different (p<0.01). The IL-18 and PDGF-β were most abundant at the lowest &lt;i&gt;Rhodomyrtus tomentosa&lt;/i&gt; doses (100-200 mg kg&lt;sup&gt;1&lt;/sup&gt; b.wt.), while they were least abundant at the 400 mg kg&lt;sup&gt;1&lt;/sup&gt; b.wt., doses. Histological analysis revealed that the highest dose of IL-18 and PDGF-β expression reduced abnormal tissue and the space between tumours, followed by several carcinoma cells that stopped growing. &lt;b&gt;Conclusion:&lt;/b&gt; &lt;i&gt;Rhodomyrtus tomentosa&lt;/i&gt; can be used as a herbal therapy to reduce the expression of PDGF-β and IL-18 (two cancer marker agents).

Keywords: Cervical cancer; IL-18; PDGF-β; Rhodomyrtus tomentosa; immunohistochemistry; lipid peroxidation; molecular therapy.