Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4

Chenggang Zhang; Xianxiong Ma; Guanxin Wei; Xiuxian Zhu; Peng Hu; Xiang Chen; Dianshi Wang; Yuan Li; Tuo Ruan; Weikang Zhang; Kaixiong Tao; Chuanqing Wu

doi:10.1016/j.isci.2022.105745

Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4

iScience. 2022 Dec 7;26(1):105745. doi: 10.1016/j.isci.2022.105745. eCollection 2023 Jan 20.

Authors

Affiliation

¹ Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei Province 430022, China.

Abstract

Centrosomal protein 120 (CEP120) is a 120 kDa centrosome protein that plays an important role in centrosome replication. Overexpression of CEP120 can lead to centrosome duplicate abnormality, which is closely associated with tumorigenesis and development. However, there are no reports on the relationship between CEP120 and tumors. In our study, overexpression of CEP120 promoted centrosome amplification in gastric cancer (GC), and the role of CEP120 in promoting GC progression was demonstrated in vitro and in vivo. We demonstrated that CEP120 promotes centrosome amplification and GC progression by promoting the expression and centrosome aggregation of the deubiquitinating enzyme USP54, maintaining the stability of PLK4 and reducing its ubiquitination degradation. In conclusion, the CEP120-USP54-PLK4 axis may play an important role in promoting centrosome amplification and GC progression, thus providing a potential therapeutic target for GC.

Keywords: Cell biology; Functional aspects of cell biology; Molecular biology.