Mouse Models of Obesity to Study the Tumor-Immune Microenvironment

Sarah Petrecca; Daniela F Quail

doi:10.1007/978-1-0716-2914-7_9

Mouse Models of Obesity to Study the Tumor-Immune Microenvironment

Methods Mol Biol. 2023:2614:121-138. doi: 10.1007/978-1-0716-2914-7_9.

Authors

Sarah Petrecca^{1

2}, Daniela F Quail^{3

4

5}

Affiliations

¹ Rosalind and Morris Goodman Cancer Institute, McGill University, Montreal, QC, Canada.
² Department of Medicine, Division of Experimental Medicine, McGill University, Montreal, QC, Canada.
³ Rosalind and Morris Goodman Cancer Institute, McGill University, Montreal, QC, Canada. daniela.quail@mcgill.ca.
⁴ Department of Medicine, Division of Experimental Medicine, McGill University, Montreal, QC, Canada. daniela.quail@mcgill.ca.
⁵ Department of Physiology, Faculty of Medicine, McGill University, Montreal, QC, Canada. daniela.quail@mcgill.ca.

PMID: 36587123
DOI: 10.1007/978-1-0716-2914-7_9

Abstract

Obesity is associated with chronic, low-grade systemic inflammation and leads to changes in the immune microenvironment of various tissues. As a result, obesity is associated with increased risk of cancer and a worse prognosis in patients. Given the prevalence of obesity worldwide, understanding the fundamental biology governing the relationship between obesity and cancer is critical. In this chapter, we describe preclinical models of obesity that can be combined with standard tumor models and techniques to study the tumor-immune microenvironment. We also discuss important considerations when planning experiments involving these models.

Keywords: Animal models; Cancer immunology; Flow cytometry; Immune microenvironment; Inflammation; Obesity; Tumor microenvironment.

MeSH terms

Animals
Disease Models, Animal
Inflammation / complications
Mice
Neoplasms*
Obesity / complications
Tumor Microenvironment