Stem cell differentiation can be modulated by the substrate topographies. Focal adhesion kinase (FAK) has been identified as a key regulator in topography-induced stem cell mechanotransduction. This chapter will describe a protocol to study the effect of FAK phosphorylation inhibition on topography-mediated stem cell differentiation. The FAK phosphorylation was inhibited using a FAK inhibitor and the effects on stem cell differentiation were examined using western blot and immunofluorescence staining.
Keywords: Focal adhesion kinase; Focal adhesion kinase phosphorylation inhibition; Immunofluorescence staining; Stem cell differentiation; Western blot.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.