Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK Phosphorylation

Methods Mol Biol. 2023:2600:297-308. doi: 10.1007/978-1-0716-2851-5_20.

Abstract

Stem cell differentiation can be modulated by the substrate topographies. Focal adhesion kinase (FAK) has been identified as a key regulator in topography-induced stem cell mechanotransduction. This chapter will describe a protocol to study the effect of FAK phosphorylation inhibition on topography-mediated stem cell differentiation. The FAK phosphorylation was inhibited using a FAK inhibitor and the effects on stem cell differentiation were examined using western blot and immunofluorescence staining.

Keywords: Focal adhesion kinase; Focal adhesion kinase phosphorylation inhibition; Immunofluorescence staining; Stem cell differentiation; Western blot.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cell Adhesion
  • Cell Differentiation
  • Focal Adhesion Kinase 1 / metabolism
  • Focal Adhesion Protein-Tyrosine Kinases / metabolism
  • Mechanotransduction, Cellular*
  • Phosphorylation

Substances

  • Focal Adhesion Protein-Tyrosine Kinases
  • Focal Adhesion Kinase 1