Mass Spectrometry of Neutral Glycosphingolipids

Abstract

This chapter describes the protocols for mass spectrometry (MS) applied to the structural characterization of neutral glycosphingolipids (GSLs) and the determination of neutral GSL contents in biological materials. The structural characterization is performed by thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS) and liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) with reversed phase separation. The content determination is carried out by LC-ESI/MS with multiple reaction monitoring (MRM). These protocols provide clues for the functions of neutral GSLs at the level of a single GSL molecular species.

Keywords: Ceramide structures; Glycan sequences; Glycosphingolipids (GSLs); Liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS); Mass spectrometry (MS); Multiple reaction monitoring (MRM); Reversed phase liquid chromatography; Thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS).