Dual-key-and-lock AIE probe for thiosulfate and Ag+ detection in mitochondria

Junrong Li; Hong Huang; Chuang Zhang; Xulang Chen; Yanjun Hu; Xiaohuan Huang

doi:10.1016/j.talanta.2022.124222

Dual-key-and-lock AIE probe for thiosulfate and Ag⁺ detection in mitochondria

Talanta. 2023 Apr 1:255:124222. doi: 10.1016/j.talanta.2022.124222. Epub 2022 Dec 27.

Authors

Junrong Li¹, Hong Huang², Chuang Zhang¹, Xulang Chen¹, Yanjun Hu¹, Xiaohuan Huang³

Affiliations

¹ Hubei Key Laboratory of Pollutant Analysis and Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, China.
² College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China.
³ Hubei Key Laboratory of Pollutant Analysis and Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, China. Electronic address: xhuang@hbnu.edu.cn.

PMID: 36586391
DOI: 10.1016/j.talanta.2022.124222

Abstract

Ag⁺ ion detection has attracted much attention due to its important role in chemical and biological processes, as well as its potential threat to the environment and human health. Herein, we firstly constructed a dual-key-and-lock sensing strategy for Ag⁺ detection based on three-component co-assembly. An aggregation-induced emission luminogen (AIEgen), namely triphenylamine-thiophene-pyridinium (abbreviated to TPA-T-Py), showed unique co-assembly capability with Ag⁺ and S₂O₃^2- in PBS buffer (pH 7.4, 0.01 M). Cell imaging further proved that mitochondria can be lit up by TPA-T-Py under the dual key stimulation, which was successfully used for Ag⁺ and S₂O₃^2- detection in vitro. In brief, we provide a promising strategy for the construction of dual-lock imaging agents with organelle-targeting ability.

Keywords: AIE; Ag(+); Dual-key-and-lock; Mitochondria-targeting; Thiosulfate.

MeSH terms

Humans
Mitochondria*
Thiosulfates*

Substances

Thiosulfates