Genomic insight into Myroides oncorhynchi sp. nov., a new member of the Myroides genus, isolated from the internal organ of rainbow trout (Oncorhynchus mykiss)

Abstract

The strain M-43^T was isolated from the Oncorhynchus mykiss from a fish farm in Mugla, Turkey. Pairwise 16S rRNA gene sequence analysis was used to identify strain M-43^T. The strain was a member of the genus Myroides sharing the highest 16S rRNA gene sequence identity levels of 98.7%, 98.3%, and 98.3% with the type strains of M. profundi D25^T, M. odoratimimus subsp. odoratimimus CCUG 39352^T and M. odoratimimus subsp. xuanwuensis DSM27251^T, respectively. A polyphasic taxonomic approach including whole genome-based analyses was employed to confirm the taxonomic provenance of strain M-43^T within the genus Myroides. The overall genome relatedness indices (OGRI) for strain M-43^T compared with its most closely related type strains M. odoratimimus subsp. xuanwuensis DSM 27251^T, M. profundi D25^T, and M. odoratimimus subsp. odoratimimus ATCC BAA-634^T, were calculated as 25.3%, 25.1%, and 25% for digital DNA-DNA hybridization (dDDH), 83.3%, 83.6%, and 83.4% for average nucleotide identity (ANI) analyses, respectively. The OGRI values between strain M-43^T and its close neighbors confirmed that the strain represents a novel species in the genus Myroides. The DNA G + C content of the strain is 33.7%. The major fatty acids are iso-C_15:0 and summed feature 9 (iso-C_17:1 ω9c and/or 10-methyl C_16:0). The predominant polar lipids are phosphatidylethanolamine, an amino-lipid and five unidentified lipids. The major respiratory quinone is MK-6. Chemotaxonomic and phylogenomic analyses of this isolate confirmed that the strain represents a novel species for which the name Myroides oncorhynchi sp. nov. is proposed, with M-43^T as the type strain (JCM 34205^T = KCTC 82265^T).

Keywords: Aquaculture; Myroides; Novel species; Overall genome-related index; Rainbow trout.