Development of QuEChERS clean-up based on EMR-lipid for simultaneous analysis of 9 mycotoxins, acaylamide and 5-Hydroxymethylfurfural in biscuit by UHPLC-MS/MS

You Long; Yousheng Huang; Mengting Zhu; Yingjie Ma; Bei Gan; YuanXing Wang; Qiang Yu; Jianhua Xie; Yi Chen

doi:10.1016/j.foodchem.2022.135265

Development of QuEChERS clean-up based on EMR-lipid for simultaneous analysis of 9 mycotoxins, acaylamide and 5-Hydroxymethylfurfural in biscuit by UHPLC-MS/MS

Food Chem. 2023 May 30:409:135265. doi: 10.1016/j.foodchem.2022.135265. Epub 2022 Dec 26.

Authors

You Long¹, Yousheng Huang², Mengting Zhu³, Yingjie Ma¹, Bei Gan⁴, YuanXing Wang¹, Qiang Yu¹, Jianhua Xie¹, Yi Chen⁵

Affiliations

¹ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
² Jiangxi Institute of Analysis and Testing, Nanchang 330029, China.
³ State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China.
⁴ Jiangxi Provincial Product Quality Supervision Testing College, Nanchang 330029, China.
⁵ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: chenyi15@ncu.edu.cn.

PMID: 36584524
DOI: 10.1016/j.foodchem.2022.135265

Abstract

A robust and sensitive analytical method for the simultaneous determination of 9 mycotoxins, AA, and 5-HMF by UHPLC-MS/MS was developed. Clean-up of the extracts was achieved by d-SPE with EMR-lipid. A new column phase (C₁₈-PFP) was selected for HPLC separation after comparison with the C₁₈ column. Finally, the method gave good linear relations with regression coefficients R² > 0.99. The recovery of all the tested compounds was within the range of 70.67 to 104.88%, and the intraday and interday relative standard deviations (RSD) were lower than 12.49. The proposed method was then applied to investigate the mycotoxins, AA and 5-HMF in 20 food samples sold in the retail market. AA and 5-HMF were widely detected, and half of the samples were found to contain at least one mycotoxin contamination. Therefore, this method is potential to be used as a convenient and effective method for the cookies product quality control in the future.

Keywords: 5-Hydroxymethylfurfural; Acrylamide; EMR-lipid; Food analysis; Mycotoxins; QuEChERS; Thermally processed food.

MeSH terms

Chromatography, High Pressure Liquid / methods
Lipids
Mycotoxins* / analysis
Tandem Mass Spectrometry* / methods

Substances

Mycotoxins
5-hydroxymethylfurfural
Lipids