Lantadene A and boswellic acid isolated from the leaves of Lantana camara L. have the potential to control phytopathogenic Fusarium species

Hlabana Alfred Seepe; Lerato Raphoko; Stephen O Amoo; Winston Nxumalo

doi:10.1016/j.heliyon.2022.e12216

Lantadene A and boswellic acid isolated from the leaves of Lantana camara L. have the potential to control phytopathogenic Fusarium species

Heliyon. 2022 Dec 15;8(12):e12216. doi: 10.1016/j.heliyon.2022.e12216. eCollection 2022 Dec.

Authors

Hlabana Alfred Seepe^{1

2

3}, Lerato Raphoko², Stephen O Amoo^{1

4

5}, Winston Nxumalo²

Affiliations

¹ Agricultural Research Council-Vegetables, Industrial and Medicinal Plants, Roodeplaat, Private Bag X293, Pretoria 0001, South Africa.
² Department of Chemistry, University of Limpopo, Private Bag X1106, Sovenga, 0727, Polokwane, South Africa.
³ Döhne Agricultural Development Institute, Plant and Crops Production Research, Private Bag X 15, Stutterheim, 4930, South Africa.
⁴ Indigenous Knowledge Systems Centre, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa.
⁵ Department of Botany and Plant Biotechnology, Faculty of Science, University of Johannesburg, P.O. Box 524, Auckland Park 2006, South Africa.

Abstract

Phytopathogenic Fusarium species are restricting factors causing diseases and yield loss in crop production. As part of exploration for pesticides from medicinal plants, this study aimed to isolate and characterize bioactive compounds from Lantana camara L. and evaluate their efficiency against Fusarium phytopathogens. Phytochemical investigation of ethyl acetate leaf extract led to separation of lantadene A (22-angeloyloxy-9-hydroxy-3-oxo-olean-12-en-28-oic acid) and boswellic acid (11-keto-β-boswellic acid). The chemical structures of the aforementioned compounds were confirmed using physical properties, spectroscopic analysis, and published data. Lantadene A exhibited significant antifungal activity against F. subglutinans, F. proliferatum, F. solani, F. graminearum, and F. semitectum with minimum inhibitory concentration (MIC) less than or equal to 0.63 mg/mL. Boswellic acid exhibited strong activity (MIC = 0.63 mg/mL) against F. subglutinans and F. semitectum. In terms of their toxicity towards Raw 264.7 cells, lantadene A and boswellic acid recorded half-maximal inhibitory concentration values of 84.2 μg/mL and 186.6 μg/mL, respectively. Both lantadene A and boswellic acid had no phytotoxic effect against seed germination and seedling root length. Lantadene A and boswellic acid have strong potential to be further investigated as lead natural fungicides (biopesticides) to control Fusarium crop diseases.

Keywords: Antifungal activity; Antimicrobial; Biopesticide; Boswellic acid; Fusarium species; Natural fungicide; Phytotoxic.