Construction of multifunctional hydrogel with metal-polyphenol capsules for infected full-thickness skin wound healing

Nanbo Liu; Shuoji Zhu; Yuzhi Deng; Ming Xie; Mingyi Zhao; Tucheng Sun; Changjiang Yu; Ying Zhong; Rui Guo; Keluo Cheng; Dehua Chang; Ping Zhu

doi:10.1016/j.bioactmat.2022.12.009

Construction of multifunctional hydrogel with metal-polyphenol capsules for infected full-thickness skin wound healing

Bioact Mater. 2022 Dec 13:24:69-80. doi: 10.1016/j.bioactmat.2022.12.009. eCollection 2023 Jun.

Authors

Nanbo Liu^{1

2}, Shuoji Zhu^{1

3

2}, Yuzhi Deng^{1

4

2}, Ming Xie^{1

2}, Mingyi Zhao^{1

2}, Tucheng Sun^{1

2}, Changjiang Yu^{1

2}, Ying Zhong⁴, Rui Guo⁵, Keluo Cheng⁴, Dehua Chang⁶, Ping Zhu^{1

4

2}

Affiliations

¹ Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, 510100, China.
² Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou, Guangdong, 510100, China.
³ University of Tokyo, Tokyo, 113-8666, Japan.
⁴ Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524001, China.
⁵ Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Centre for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.
⁶ University of Tokyo Hospital Department of Cell Therapy in Regenerative Medicine, Tokyo, 113-8666, Japan.

Abstract

Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds or even severe tissue damage. In this study, we developed a controlled-release antibacterial composite hydrogel system that can promote wound angiogenesis and inhibit inflammation by sustained releasing Cu-Epigallocatechin-3-gallate (Cu-EGCG) nano-capsules. The prepared SilMA/HAMA/Cu-EGCG hydrogel showed an obvious inhibitory effect on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). It could also promote the proliferation and migration of L929 fibroblasts. In vivo full-thickness infected wound healing experiments confirmed the angiogenesis and inflammation regulating effect. Accelerate collagen deposition and wound healing speed were also observed in the SilMA/HAMA/Cu-EGCG hydrogel treated group. The findings of this study show the great potential of this controlled-release antibacterial composite hydrogel in the application of chronic wound healing.

Keywords: Copper; Epigallocatechin-3-gallate; Infected wound healing; Metal polyphenol network.