We collected >40,000 mosquitoes from 5 provinces in South Africa during 2011-2018 and screened for zoonotic flaviviruses. We detected West Nile virus in mosquitoes from conservation and periurban sites and potential new mosquito vectors; Banzi virus was rare. Our results suggest flavivirus transmission risks are increasing in South Africa.
Keywords: Banzi virus; Culex; Culicidae; South Africa; West Nile virus; disease vectors; flavivirus; vector-borne infections; viruses; zoonoses.