Isolation and characterization of wheat ice recrystallisation inhibition gene promoter involved in low temperature and methyl jasmonate responses

Yanan Jin; Xihan Ding; Jianbo Li; Zhifu Guo

doi:10.1007/s12298-022-01257-6

Isolation and characterization of wheat ice recrystallisation inhibition gene promoter involved in low temperature and methyl jasmonate responses

Physiol Mol Biol Plants. 2022 Dec;28(11-12):1969-1979. doi: 10.1007/s12298-022-01257-6. Epub 2022 Dec 6.

Authors

Yanan Jin^#¹, Xihan Ding², Jianbo Li^{3

4}, Zhifu Guo^#²

Affiliations

¹ College of Life Science and Food Engineering, Inner Mongolia Minzu University, 536 Huolinhe Street West, Tongliao City, 028043 Inner Mongolia China.
² College of Bioscience and Biotechnology, Shenyang Agricultural University, 120 Dongling Street, Shenyang City, 110866 Liaoning China.
³ College of Agriculture, Inner Mongolia Minzu University, Tongliao, 028043 China.
⁴ Engineering Technology Research Center of Forage Crops in Inner Mongolia, Inner Mongolia Minzu University, Tongliao, 028043 China.

^# Contributed equally.

Abstract

It is well known that plant growth, development, survival and geographical distribution are constrained by extreme climatic conditions, especially extreme low temperature. Under cold stress, cold-inducible promoters were identified as important molecular switches to transcriptionally regulate the initiation of genes associated with cold acclimation processes and enhance the adaptability of plants to cold stimulation. Wheat (Triticum aestivum L.) is one of the most dominating food crops in the world, and wheat crops are generally overwintering with strong cold resistance. Our previous study already proved that heterologous expression of wheat ice recrystallization inhibition (IRI) genes enhanced freezing tolerance in tobacco. However, the upstream regulatory mechanisms of TaIRI are ambiguous. In this study, the space-time specific expression of TaIRI genes in wheat was analyzed by quantitative real-time PCR (qRT-PCR), and results showed that the expression of TaIRI in all tissues was cold-induced and accelerate by exogenous methyl jasmonate (MeJA). Three promoters of TaIRI genes were isolated from wheat genome, and various 5'-deletion fragments of TaIRIp were integrated into β-glucuronidase (GUS) within vector pCAMBIA1301. The promoter activity of TaIRI genes was determined through transient expression system of tobacco and stable expression of Arabidopsis thaliana. Results revealed that the GUS activity were significantly strengthened by cold and MeJA treatments. This study will provide insights into elucidating the transcription-regulatory mechanism of IRI proteins responding to low temperature.

Keywords: Cold; Methyl jasmonate; TaIRI promoter; Wheat.

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