Evaluating the value of anti-SARS-CoV-2 antibody detection and neutralizing responses with euvirus: A population of 10776 close contacts in the epidemic of Fujian

Yongbin Zeng; Caorui Lin; Can Liu; Chun Huang; Falin Chen; Yingping Cao; Siying Wu; Donghong Wei; Zhong Lin; Yali Zhang; Ling Zhang; Jing Teng; Zishun Li; Guolin Hong; Tianci Yang; Huiming Ye; Haijian Tu; Yupeng Xiao; Lishan Huang; Jiawei Zhang; Tianbin Chen; Jinming Li; Fusui Ji; Qishui Ou

doi:10.1016/j.cca.2022.12.017

Evaluating the value of anti-SARS-CoV-2 antibody detection and neutralizing responses with euvirus: A population of 10776 close contacts in the epidemic of Fujian

Clin Chim Acta. 2023 Jan 15:539:237-243. doi: 10.1016/j.cca.2022.12.017. Epub 2022 Dec 23.

Authors

Yongbin Zeng¹, Caorui Lin¹, Can Liu¹, Chun Huang², Falin Chen³, Yingping Cao⁴, Siying Wu⁵, Donghong Wei⁵, Zhong Lin⁶, Yali Zhang⁷, Ling Zhang⁸, Jing Teng⁹, Zishun Li¹⁰, Guolin Hong¹¹, Tianci Yang¹², Huiming Ye¹³, Haijian Tu¹⁴, Yupeng Xiao¹⁵, Lishan Huang¹⁶, Jiawei Zhang¹, Tianbin Chen¹, Jinming Li¹⁷, Fusui Ji¹⁸, Qishui Ou¹⁹

Affiliations

¹ Department of Laboratory Medicine, Gene Diagnosis Research Center, The First Affiliated Hospital, Fujian Medical University, Fuzhou, PR China; Fujian Key Laboratory of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, PR China.
² Fujian Medical Association, Fuzhou, PR China.
³ Department of Clinical Laboratory, Fujian Provincial Hospital, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, PR China.
⁴ Department of Laboratory Medicine, Fujian Medical University Union Hospital, Fuzhou, PR China.
⁵ Department of Epidemiology and Health Statistics, School of Public Health, Fujian Medical University, Fuzhou, PR China.
⁶ Fujian Provincial Center for Disease Control and Prevention, Fuzhou, PR China.
⁷ The State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Center of Biologic Products, School of Public Health and School of Life Science, Xiamen University, Xiamen, PR China.
⁸ Department of Laboratory Medicine, Land Force No.73 Group Army Hospital of PLA, Xiamen, PR China.
⁹ Department of Laboratory Medicine, Beijing University of Chinese Medicine Xiamen Hospital, Xiamen, PR China.
¹⁰ Department of Laboratory Medicine, The Third Hospital of Xiamen, Xiamen, PR China.
¹¹ Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen Key Laboratory of Genetic Testing, School of Medicine, Xiamen University, Xiamen, PR China.
¹² Center of Clinical Laboratory, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, PR China.
¹³ Department of Clinical Laboratory, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, PR China.
¹⁴ Department of Laboratory Medicine, Affiliated Hospital of Putian University, Putian, PR China.
¹⁵ Department of Clinical Laboratory, Putian Municipal First Hospital, Putian, PR China.
¹⁶ Fujian Provincial Healthcare Center, Fuzhou, PR China.
¹⁷ National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, Beijing, PR China. Electronic address: jmli@nccl.org.cn.
¹⁸ Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Science, PR China. Electronic address: jifusui0367@bjhmoh.cn.
¹⁹ Department of Laboratory Medicine, Gene Diagnosis Research Center, The First Affiliated Hospital, Fujian Medical University, Fuzhou, PR China; Fujian Key Laboratory of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, PR China. Electronic address: ouqishui@fjmu.edu.cn.

Abstract

Background: Nucleic acid detection represents limitations due to its false-negative rate and technical complexity in the COVID-19 pandemic. Anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody tests are widely spread all over the world presently. However, there is no report on the effectiveness of anti-SARS-CoV-2 antibody testing methods in China.

Methods: We gathered 10776 serum samples from close contacts of the SARS-CoV-2 infections in Fujian of China and used 2 chemiluminescence immunoassays (Wantai Bio., Yahuilong Bio.) and 2 lateral flow immunoassays (Lizhu Bio. and Dongfang Bio.) to perform the anti-SARS-CoV-2 antibody tests in China.

Results: The 4 antibody tests have great diagnostic value for infected or uninfected, especially in the neutralizing antibodies tests, the AUC can reach 0.939 (Wantai Bio.) and 0.916 (Yahuilong Bio.). Furthermore, we used pseudoviruses and euvirus neutralization assay to validate the effectiveness of these antibody test, the results of pseudoviruses neutralization assay or euvirus neutralization assay shows a considerable correlation with the 4 antibody detection respectively, particularly in euvirus neutralization assay, neutralizing antibodies detected by Wantai Bio. or Yahuilong Bio., the correlation can get the level of 0.93 or 0.82.

Conclusions: The findings of this study demonstrate that the detections of antibodies have profound value in the diagnosis of COVID-19.

Keywords: Antibody test; COVID-19; Chemiluminescence immunoassays; Euvirus neutralization assay; Lateral flow immunoassays; Pseudoviruses neutralization assay; SARS-CoV-2.

MeSH terms

Antibodies, Neutralizing
Antibodies, Viral
COVID-19* / diagnosis
Humans
Pandemics
SARS-CoV-2

Substances

Antibodies, Viral
Antibodies, Neutralizing