Colorimetric detection of biothiols and Hg2+ based on the peroxidase-like activity of GTP

Na Huang; Dan Yang; Huanhuan Chen; Yu Xiao; Jiahui Wen; Yijuan Long; Huzhi Zheng

doi:10.1016/j.saa.2022.122263

Colorimetric detection of biothiols and Hg²⁺ based on the peroxidase-like activity of GTP

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Apr 5:290:122263. doi: 10.1016/j.saa.2022.122263. Epub 2022 Dec 20.

Authors

Na Huang¹, Dan Yang¹, Huanhuan Chen¹, Yu Xiao¹, Jiahui Wen¹, Yijuan Long¹, Huzhi Zheng²

Affiliations

¹ College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China.
² College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China. Electronic address: zhenghz@swu.edu.cn.

PMID: 36571862
DOI: 10.1016/j.saa.2022.122263

Abstract

Guanosine-5'-triphosphate (GTP) not only plays a key role in a majority of cellular processes but also be proposed as a peroxidase-like mimic. Compared with nanozymes, GTP shows good tolerance under harsh conditions, which can be used to construct an easy colorimetric analysis for the detection of biomolecules. Here, on the basis of the peroxidase-like activity of GTP which can catalyze the oxidation of 3,3',5,5'-tetramethyl benzidine dihydrochloride (TMB), colorimetric sensing was established for biothiols and Hg²⁺. Biothiols reduced the oxTMB back to colorless TMB, and Hg²⁺ restored the formation of oxTMB, leading to the recovery of color. This method not only provides a platform for the detection of metal ions and biothiols, but also shows that GTP has great potential for analytical detection.

Keywords: Biothiols; GTP; Hg(2+); Peroxidase-like activity.

MeSH terms

Colorimetry* / methods
Hydrogen Peroxide
Mercury*
Oxidoreductases
Peroxidase
Peroxidases

Substances

Peroxidase
Peroxidases
Oxidoreductases
Mercury
Hydrogen Peroxide