Bioremediation of multifarious pollutants using laccase immobilized on magnetized and carbonyldiimidazole-functionalized cellulose nanofibers

Kavita Sharma; Preeti Tewatia; Manpreet Kaur; Deepak Pathania; Fawzi Banat; Gaurav Rattan; Sonal Singhal; Anupama Kaushik

doi:10.1016/j.scitotenv.2022.161137

Bioremediation of multifarious pollutants using laccase immobilized on magnetized and carbonyldiimidazole-functionalized cellulose nanofibers

Sci Total Environ. 2023 Mar 15:864:161137. doi: 10.1016/j.scitotenv.2022.161137. Epub 2022 Dec 23.

Authors

Kavita Sharma¹, Preeti Tewatia¹, Manpreet Kaur¹, Deepak Pathania², Fawzi Banat³, Gaurav Rattan⁴, Sonal Singhal⁵, Anupama Kaushik⁶

Affiliations

¹ Energy Research Centre, Panjab University, Chandigarh, India.
² Department of Environmental Sciences, Central University of Jammu, J&K, India; Department of Chemistry, Sardar Patel University Mandi, Himachal Pradesh 175001, India.
³ Dept of Chemical Engineering, Khalifa University, Abu Dhabi, United Arab Emirates.
⁴ Dr. SSB University Institutes of Chemical Engineering and Technology, Panjab University, Chandigarh, India.
⁵ Department of Chemistry, Panjab University, Chandigarh, India.
⁶ Dr. SSB University Institutes of Chemical Engineering and Technology, Panjab University, Chandigarh, India. Electronic address: anupamachem@gmail.com.

PMID: 36566870
DOI: 10.1016/j.scitotenv.2022.161137

Abstract

An easily recyclable biocatalyst (Lac@CDI-MCNFs) was synthesized by immobilizing laccase on rice straw-derived carbonyldiimidazole mediated magnetized cellulose nanofibers (MCNFs). Lac@CDI-MCNFs were utilized for bioremediation of cefixime antibiotic (CT), carbofuran pesticide (CF) and safranin O dye (SO) via oxidation-reduction reactions in wastewater. MCNFs provided enhanced pH, temperature and storage stability to laccase and allowed reusability for up to 25 cycles with mere 20 % decline in efficacy. The Lac@CDI-MCNFs effectively degraded 98.2 % CT and 96.8 % CF into benign metabolites within 20 h and completely degraded SO in just 7 h. Response surface modelling (RSM) was employed based on the Box Behnken Design to evaluate the effect of various parameters i.e. pH, catalyst dosage and the pollutants concentration which was further validated with experimental studies. The degradation products were identified using LCMS, which allowed the degradation pathway of the pollutants to be determined. The degradation of all pollutants followed first- order kinetics with rate constants of 0.1775, 0.0832 and 0.958 h^-1 and half-life of 3.9, 5.0 and 0.723 h for CT, CF and SO, respectively. Lac@CDI-MCNFs was demonstrated to be an effective catalyst for the degradation of multifarious pollutants.

Keywords: Carbofuran (CF); Carbonyldiimidazole; Cefixime trihydrate (CT); Degradation; Laccase immobilized magnetic cellulose nanofibers; Rice straw; Safranin O (SO).

MeSH terms

Biodegradation, Environmental
Cellulose
Environmental Pollutants*
Enzymes, Immobilized / metabolism
Laccase / metabolism
Nanofibers*

Substances

Cellulose
Enzymes, Immobilized
Laccase
Environmental Pollutants