A highly efficient molecularly imprinted fluorescence sensor for assessing whole wheat grains by the rapid and sensitive detection of alkylresorcinols

Abstract

To differentiate whole wheat foods from refined wheat foods is still challenging grain industry and confusing consumers. Alkylresorcinols (ARs), as biomarkers of whole wheat grains, can serve for assessing the authenticity of whole wheat foods. Herein, a highly efficient fluorescence sensing platform (CDs@MIP) for rapid and sensitive analysis of ARs was explored, using carbon dots (CDs) as fluorophores and 5-heneicosylresorcinol (C21:0 AR) as template molecules embedded in a molecularly imprinted polymer (MIP) coating. Benefiting from the specific cavities in the probe and a photo-induced electron transfer effect, the fluorescence intensity of CDs@MIP was significantly quenched in the presence of C21:0 AR, exhibiting a superior binding efficiency and selectivity. As a result, the fabricated optical sensor delivered a wide linear range of C21:0 AR from 0.015 to 60 μg mL^-1 with an ultralow detection limit of 4 ng mL^-1. It was noteworthy that the sensor was successfully applied for the rapid detection of C21:0 AR in commercial whole-wheat foods as well as visualization analysis on the test paper, comprehensively validating the practicality and efficacy of CDs@MIP based fluorescence assay. The study provides a rapid and sensitive detection method of C21:0 AR, paving a new way for guiding grain industry to effectively qualify the authenticity and to quantify the content of whole wheat in wheat-based foods.

Keywords: Alkylresorcinols; Fluorescence sensor; Molecularly imprinted polymer; Optosensing; Whole wheat foods.