Associations between hair trace mineral concentrations and the occurrence of treponeme-associated hoof disease in elk (Cervus canadensis)

Steven N Winter; Maria Del Pilar Fernandez; Kyle R Taylor; Margaret A Wild

doi:10.1186/s12917-022-03547-3

Associations between hair trace mineral concentrations and the occurrence of treponeme-associated hoof disease in elk (Cervus canadensis)

BMC Vet Res. 2022 Dec 23;18(1):446. doi: 10.1186/s12917-022-03547-3.

Authors

Steven N Winter^{1

2}, Maria Del Pilar Fernandez², Kyle R Taylor^{1

3}, Margaret A Wild⁴

Affiliations

¹ Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164, USA.
² Paul G. Allen School for Global Health, Washington State University, Pullman, WA, 99164, USA.
³ Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, WA, 99164, USA.
⁴ Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164, USA. margaret.wild@wsu.edu.

Abstract

Background: Trace minerals are important for animal health. Mineral deficiency or excess can negatively affect immune function, wound healing, and hoof health in domestic livestock, but normal concentrations and health impairment associated with mineral imbalances in wild animals are poorly understood. Treponeme-associated hoof disease (TAHD) is an emerging disease of free-ranging elk (Cervus canadensis) in the U.S. Pacific Northwest. Selenium and copper levels identified in a small number of elk from areas where TAHD is established (i.e., southwestern Washington) suggested a mineral deficiency may have increased susceptibility to TAHD. Our objectives were to determine trace mineral concentrations using hair from elk originating in TAHD affected areas of Washington, California, Idaho, and Oregon and assess their associations with the occurrence of the disease.

Results: We identified limited associations between TAHD occurrence and severity with hair mineral concentrations in 72 free-ranging elk, using Firth's logistic regression and multinomial regression models. We found consistent support for a priori hypotheses that selenium concentration, an important mineral for hoof health, is inversely associated with the occurrence of TAHD. Less consistent support was observed for effects of other minerals previously associated with hoof health (e.g., copper or zinc) or increased disease risk from potential toxicants.

Conclusion: Trace mineral analysis of hair is a non-invasive sampling technique that offers feasibility in storage and collection from live animals and carcasses. For some minerals, levels in hair correlate with visceral organs that are challenging to obtain. Our study using hair collected opportunistically from elk feet submitted for diagnostic investigations provides a modest reference of hair mineral levels in elk from the U.S. Pacific Northwest that may be useful in future determination of reference ranges. Although our results revealed high variability in mineral concentrations between elk, consistent relationship of possibly low selenium levels and TAHD suggest that further investigations are warranted.

Keywords: Cervus canadensis; Elk; hair; trace minerals; treponeme-associated hoof disease.

MeSH terms

Animals
Copper
Deer*
Hair
Hoof and Claw*
Selenium*
Trace Elements*
Treponema

Substances

Trace Elements
Copper
Selenium