Sanwei sandalwood decoction ameliorates acute ischemiareperfusion injury in rats by modulating myocyte electrophysiological characteristics

Quan Wan; Yu Wang; Ming Yong; PengFei Hu; Che Ge Le Liang; Xiang Jun Yang; Xiao Zhao; Dan San; Ting Ting Bai; La Ga Tong; Jingbo Zhai; Ming Zhao; QingShan Zhang

doi:10.1016/j.biopha.2022.114103

Sanwei sandalwood decoction ameliorates acute ischemiareperfusion injury in rats by modulating myocyte electrophysiological characteristics

Biomed Pharmacother. 2023 Feb:158:114103. doi: 10.1016/j.biopha.2022.114103. Epub 2022 Dec 21.

Authors

Quan Wan¹, Yu Wang², Ming Yong¹, PengFei Hu¹, Che Ge Le Liang¹, Xiang Jun Yang¹, Xiao Zhao¹, Dan San¹, Ting Ting Bai¹, La Ga Tong¹, Jingbo Zhai³, Ming Zhao⁴, QingShan Zhang⁵

Affiliations

¹ Affiliated Hospital of Inner Mongolia Minzu University, Tongliao, Inner Mongolia, PR China; Inner Mongolia Minzu University, Tongliao, Inner Mongolia, P.R. China; Institute of Cardiovascular Diseases of Combination of Mongolian and Western Medicine of Inner Mongolia Minzu University.
² Inner Mongolia Minzu University, Tongliao, Inner Mongolia, P.R. China; Institute of Cardiovascular Diseases of Combination of Mongolian and Western Medicine of Inner Mongolia Minzu University.
³ Inner Mongolia Minzu University, Tongliao, Inner Mongolia, P.R. China; Key Laboratory of Zoonose Prevention and Control at Universities of Inner Mongolia Autonomous Region, Tongliao 028000, PR China.
⁴ Affiliated Hospital of Inner Mongolia Minzu University, Tongliao, Inner Mongolia, PR China; Inner Mongolia Minzu University, Tongliao, Inner Mongolia, P.R. China; Institute of Cardiovascular Diseases of Combination of Mongolian and Western Medicine of Inner Mongolia Minzu University. Electronic address: langzhe73@163.com.
⁵ Affiliated Hospital of Inner Mongolia Minzu University, Tongliao, Inner Mongolia, PR China; Inner Mongolia Minzu University, Tongliao, Inner Mongolia, P.R. China; Institute of Cardiovascular Diseases of Combination of Mongolian and Western Medicine of Inner Mongolia Minzu University. Electronic address: nmzqs@163.com.

PMID: 36563556
DOI: 10.1016/j.biopha.2022.114103

Abstract

Sanwei sandalwood decoction (SWTX) is a classical Chinese medicine formula and clinically effective treatment for coronary heart disease, including myocardial ischemia/reperfusion (I/R) injury. Because the treatment mechanism of SWTX in I/R injury remains obscure, we intended to analyze the potential cardioprotective effects of SWTX in rats with myocardial I/R injury. Our research revealed that SWTX prolonged ventricular conduction time in a dose-dependent manner. While SWTX significantly delayed left ventricular signal conduction velocity, it had no effect on left atrial conduction velocity. Under sinus conditions, low SWTX concentrations reduced left ventricular conduction dispersion, while high concentrations increased conduction dispersion. SWTX also prolonged the QRS interval, APD30/50/90, and ERP. In whole-cell patch clamp experiments on myocytes, Ito and Ikr were inhibited by SWTX. While SWTX had no effect on I_Na, the activation curve for Nav1.5 was left-shifted. Finally, SWTX reduced the probability of ventricular fibrillation and suppressed early and late depolarization in an acute I/R injury rat model. These findings shed light on the mechanism by which SWTX alleviates myocardial I/R injury.

Keywords: Electrophysiological characteristics; Ischemia/reperfusion injury; Sanwei sandalwood decoction.

MeSH terms

Animals
Arrhythmias, Cardiac / complications
Electrophysiological Phenomena
Heart Ventricles
Muscle Cells
Myocardial Reperfusion Injury*
Rats
Santalum*