Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves

Alexandra Buckley; Lauren Crawford; Kyle Hoffman; Shollie Falkenberg

doi:10.3390/v14122809

Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves

Viruses. 2022 Dec 16;14(12):2809. doi: 10.3390/v14122809.

Authors

Alexandra Buckley¹, Lauren Crawford², Kyle Hoffman¹, Shollie Falkenberg³

Affiliations

¹ Virus and Prion Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA 50010, USA.
² Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA 50010, USA.
³ Ruminant Disease and Immunology Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA 50010, USA.

Abstract

Senecavirus A (SVA) is a causative agent for vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases of swine including foot-and-mouth disease (FMD). Recently, it was reported that buffalo in Guangdong, China were experiencing clinical symptoms similar to FMD including mouth ulcers and lameness tested positive for SVA. The objective of this study was to determine the susceptibility of cattle (Bos taurus) to SVA infection. Initial in vitro work using the PrimeFlow assay demonstrated that bovine cell lines and peripheral blood mononuclear cells from cattle were susceptible to SVA infection. Subsequently, six colostrum-deprived Holstein calves were challenged with SVA intranasally. No vesicular lesions were observed after challenge. Serum, oral, nasal, and rectal swabs tested for SVA nucleic acid did not support significant viral replication and there was no evidence of seroconversion. Therefore, demonstrating cattle from this study were not susceptible to experimental SVA infection.

Keywords: Senecavirus A; cattle; susceptibility; vesicular disease.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cattle
Cell Line
Colostrum
Female
Foot-and-Mouth Disease*
Leukocytes, Mononuclear
Picornaviridae Infections*
Picornaviridae*
Pregnancy
Swine
Swine Diseases*

Supplementary concepts

Senecavirus A

Grants and funding

This study was supported by USDA-ARS. L.C. and K.H. were supported in part by an appointment to the USDA-ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and USDA. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA, DOE, or ORISE.