Isolation and Degradation Characteristics of PBAT Film Degrading Bacteria

Rehemanjiang Wufuer; Wenfeng Li; Shuzhi Wang; Jia Duo

doi:10.3390/ijerph192417087

Isolation and Degradation Characteristics of PBAT Film Degrading Bacteria

Int J Environ Res Public Health. 2022 Dec 19;19(24):17087. doi: 10.3390/ijerph192417087.

Authors

Rehemanjiang Wufuer^{1

2}, Wenfeng Li^{1

2}, Shuzhi Wang^{1

2}, Jia Duo^{1

2}

Affiliations

¹ State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
² Xinjiang Key Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.

Abstract

In recent years, PBAT (polybutylene adipate-co-terephthalate) mulch has become one of the most commonly used biodegradable mulching films. In this paper, five potential strains of PBAT film degrading bacteria were screened from the soil sample using PBAT film as the sole carbon source. A highly efficient PBAT degrading strain JZ1 was isolated by comparing the degradation performance of PBAT mulching film identified as Peribacillus frigoritolerans S2313 by 16S rDNA sequence analysis. The capacity of the strain to degrade PBAT film was optimized by adjusting the cultivation conditions such as nitrogen source, pH, and inoculum volume. After 8 weeks of cultivation, the actual degradation rate of the strain to PBAT mulch film reached 12.45%. SEM (scanning electron microscopy) coupled with EDX (energy dispersive spectroscopy) analysis showed that microbial degradation is an oxidation process and is mainly due to the amorphous regions of the PBAT film. The biodegradation of PBAT film by Peribacillus frigoritolerans may provide a promising method for regulating the degradation progress of PBAT film in the farmlands.

Keywords: PBAT mulching film; cultivation; degradation rate; nitrogen source.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillaceae*
Bacteria
Biodegradation, Environmental
Farms
Microscopy, Electron, Scanning
Motion Pictures
Polyesters* / chemistry

Substances

Polyesters

Grants and funding

This research was supported by the West Light Foundation of the Chinese Academy of Sciences (2018-XBQNXZ-B-014) and the Science and Technology Department of Xinjiang Uyghur Autonomous Region (2022D04063, 2017B03014-1, 2).