A bioactive film with two concentrations of sage seed gum (SSG) (1 % and 1.5 %) incorporating 2 % and 4 % Zataria multiflora essential oil (ZMEO) nanoemulsion was developed. Microbiological evaluation, including disc diffusion and time-kill tests, as well as mechanical and chemical characteristics namely film thickness, water-solubility, water vapor permeability, tensile strength and elongation at break, scanning electron microscopy (SEM), Fourier transform-infrared (FT-IR) analysis, antioxidant activity, and color analysis, were examined. Results showed that the antimicrobial properties of SSG films incorporating ZMEO nanoemulsion increased significantly (P < 0.05) by the multiplication of essential oil concentration. The films with 1 % SSG-4 % ZMEO showed acceptable antioxidant properties (∼65 %), and improved physical properties (508 % thickness increase, 56.63 % water solubility decrease, and 36.85 % water vapor permeability decrease), whereas tensile strength decreased only 29.8 %, and elongation increased 115 %. According to the results, SSG-ZMEO film may have positive potential impacts on increasing the shelf-life of foodstuffs.
Keywords: Bioactive film; Essential oil; Nanoemulsion; Sage seed gum; Zataria multiflora.
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