A peptide fraction from hardened common beans (Phaseolus vulgaris) induces endothelium-dependent antihypertensive and renal effects in rats

Juliana Vila Verde Ribeiro; Daniel Graziani; Jhulle Horrane Moreira Carvalho; Michelle Mendanha Mendonça; Lara Marques Naves; Helton Freires Oliveira; Hericles Mesquita Campos; Maria Clorinda Soares Fioravanti; Lilian Fernanda Pacheco; Patricia Maria Ferreira; Gustavo Rodrigues Pedrino; Paulo César Ghedini; Kátia Flávia Fernandes; Karla de Aleluia Batista; Carlos Henrique Xavier

doi:10.1016/j.crfs.2022.100410

A peptide fraction from hardened common beans (Phaseolus vulgaris) induces endothelium-dependent antihypertensive and renal effects in rats

Curr Res Food Sci. 2022 Dec 5:6:100410. doi: 10.1016/j.crfs.2022.100410. eCollection 2023.

Authors

Juliana Vila Verde Ribeiro¹, Daniel Graziani¹, Jhulle Horrane Moreira Carvalho¹, Michelle Mendanha Mendonça¹, Lara Marques Naves², Helton Freires Oliveira³, Hericles Mesquita Campos⁴, Maria Clorinda Soares Fioravanti³, Lilian Fernanda Pacheco⁵, Patricia Maria Ferreira¹, Gustavo Rodrigues Pedrino², Paulo César Ghedini⁴, Kátia Flávia Fernandes⁶, Karla de Aleluia Batista⁷, Carlos Henrique Xavier¹

Affiliations

¹ Systems Neurobiology Laboratory, Institute of Biological Sciences, Federal University of Goiás, Brazil.
² Center of Neuroscience and Cardiovascular Research, Institute of Biological Sciences, Federal University of Goiás, Brazil.
³ Molecule, Cell and Tissue Analysis Laboratory, School of Veterinary and Animal Science, Federal University of Goiás, Brazil.
⁴ Biochemical and Molecular Pharmacology Laboratory, Institute of Biological Sciences, Federal University of Goiás, Brazil.
⁵ State Univeristy of Goiás, Brazil.
⁶ Polymer Chemistry Laboratory, Institute of Biological of Sciences, Federal University of Goiás, Brazil.
⁷ Federal Institute for Education, Science and Technology of Goiás, Goiania Oeste Campus, Brazil.

Abstract

Beans reached the research spotlight as a source of bioactive compounds capable of modulating different functions. Recently, we reported antioxidant and oxidonitrergic effect of a low molecular weight peptide fraction (<3 kDa) from hardened bean (Phaseolus vulgaris) in vitro and ex vivo, which necessitate further in vivo assessments. This work aimed to evaluate the hypotensive effect and the involved physiological mechanisms of the hardened common bean peptide (Phaseolus vulgaris) in normotensive (Wistar) and hypertensive (SHR) animals. Bean flour was combined with a solution containing acetonitrile, water and formic acid (25: 24: 1). Protein extract (PV3) was fractioned (3 kDa membrane). We assessed PV3 effects on renal function and hemodynamics of wistar (WT-normotensive) and spontaneously hypertensive rats (SHR) and measured systemic arterial pressure and flow in aortic and renal beds. The potential endothelial and oxidonitrergic involvements were tested in isolated renal artery rings. As results, we found that PV3: I) decreased food consumption in SHR, increased water intake and urinary volume in WT, increased glomerular filtration rate in WT and SHR, caused natriuresis in SHR; II) caused NO- and endothelium-dependent vasorelaxation in renal artery rings; III) reduced arterial pressure and resistance in aortic and renal vascular beds; IV) caused antihypertensive effects in a dose-dependent manner. Current findings support PV3 as a source of bioactive peptides and raise the potential of composing nutraceutical formulations to treat renal and cardiovascular diseases.

Keywords: ABF, Aortic blood flow; AVR, Aortic vascular resistance; Bioactive peptides; Common beans; GFR, Glomerular filtration rate; HTC, Hard-to-Cook effects; Hard-to-cook; Hydroelectrolytic balance; Hypertension; L-NAME, nitroarginine methyl ester; NO, Nitric oxide; PV3, Phaseolus vulgaris extract with peptides smaller than 3 kDa; Phaseolus vulgaris; RBF, Renal blood flow; RVR, Renal vascular resistance; Renal function; SHR, Spontaneously hypertensive rat; WT, Wistar rat.