An oral ratiometric NIR-II fluorescent probe for reliable monitoring of gastrointestinal diseases in vivo

Tuanwei Li; Kaili Cao; Xiaohu Yang; Yongyang Liu; Xingyu Wang; Feng Wu; Guangcun Chen; Qiangbin Wang

doi:10.1016/j.biomaterials.2022.121956

An oral ratiometric NIR-II fluorescent probe for reliable monitoring of gastrointestinal diseases in vivo

Biomaterials. 2023 Feb:293:121956. doi: 10.1016/j.biomaterials.2022.121956. Epub 2022 Dec 15.

Authors

Tuanwei Li¹, Kaili Cao², Xiaohu Yang¹, Yongyang Liu², Xingyu Wang², Feng Wu², Guangcun Chen³, Qiangbin Wang⁴

Affiliations

¹ CAS Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory of Functional Molecular Imaging Technology, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
² CAS Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory of Functional Molecular Imaging Technology, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China; School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, 230026, China.
³ CAS Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory of Functional Molecular Imaging Technology, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China; School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, 230026, China. Electronic address: gcchen2011@sinano.ac.cn.
⁴ CAS Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory of Functional Molecular Imaging Technology, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China; School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, 230026, China; College of Materials Sciences and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: qbwang2008@sinano.ac.cn.

PMID: 36543049
DOI: 10.1016/j.biomaterials.2022.121956

Abstract

Early monitoring of gastrointestinal diseases via orally delivered NIR-II ratiometric fluorescent probes represents a promising noninvasive diagnostic modality, but is challenging due to the limitation of harsh digestive environment. Here, we report a single-component NIR-II ratiometric molecular nanoprobe (LC-1250 NP) to monitor gastrointestinal disease with high specificity to its biomarker H₂O₂ via oral administration. LC-1250 NP displays stable fluorescence in the channel of 1250 long-pass (F_1250LP) before and after the gastrointestinal disease detection as the reference, while it presents significantly enhanced fluorescence signal in the response channel of 1150 nm short-pass (F_1150SP) in diseased gastrointestinal environment due to the intramolecular cyclization of LC-1250 molecules activated by H₂O₂. The fluorescence ratio (F_1150SP/F_1250LP) increases linearly with the concentration of H₂O₂ with a low detection limit of 20 nM. Therefore, when delivered orally, LC-1250 NP can accurately map the diseased areas and surmount the false-positive interference from biological heterogeneity by NIR-II ratiometric fluorescence imaging, providing sensitive and reliable evaluation for the progress of gastroenteritis.

Keywords: Gastrointestinal diseases; Hydrogen peroxide; NIR-II organic Dye; Oral administration; Ratiometric fluorescence imaging.

MeSH terms

Fluorescence
Fluorescent Dyes*
Gastrointestinal Diseases*
Humans
Hydrogen Peroxide
Nanotechnology

Substances

Fluorescent Dyes
Hydrogen Peroxide