CyTOF protocol for immune monitoring of solid tumors from mouse models

Sarah M Shin; Alexei Hernandez; Erin Coyne; Kabeer Munjal; Nicole E Gross; Soren Charmsaz; Xuan Yuan; Hongqui Yang; Won Jin Ho

doi:10.1016/j.xpro.2022.101949

CyTOF protocol for immune monitoring of solid tumors from mouse models

STAR Protoc. 2023 Mar 17;4(1):101949. doi: 10.1016/j.xpro.2022.101949. Epub 2022 Dec 19.

Authors

Sarah M Shin¹, Alexei Hernandez¹, Erin Coyne¹, Kabeer Munjal¹, Nicole E Gross¹, Soren Charmsaz¹, Xuan Yuan², Hongqui Yang², Won Jin Ho³

Affiliations

¹ Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA.
² Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA; Mass Cytometry Facility at Johns Hopkins University, Baltimore, MD 21287, USA.
³ Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21287, USA; Mass Cytometry Facility at Johns Hopkins University, Baltimore, MD 21287, USA; Convergence Institute, Johns Hopkins University, Baltimore, MD 21287, USA. Electronic address: wjho@jhmi.edu.

Abstract

Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects. For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021).¹.

Keywords: Antibody; Cancer; Flow Cytometry/Mass Cytometry; Immunology.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Mice
Monitoring, Immunologic
Neoplasms* / diagnosis

Abstract

Publication types

MeSH terms

Grants and funding