Identification of a Multidrug Resistant Pseudomonas aeruginosa Isolate Harboring Infrequent Red Fluorescence Plasmid from COPD Patient

Qianglin Zeng; Xiting Yang; Heyue Li; Jiajie Zhang; Yamei Zhang; Hui Zhou; Kelei Zhao

doi:10.2147/IDR.S383820

Identification of a Multidrug Resistant Pseudomonas aeruginosa Isolate Harboring Infrequent Red Fluorescence Plasmid from COPD Patient

Infect Drug Resist. 2022 Dec 13:15:7301-7305. doi: 10.2147/IDR.S383820. eCollection 2022.

Authors

Qianglin Zeng¹, Xiting Yang¹, Heyue Li², Jiajie Zhang¹, Yamei Zhang¹, Hui Zhou¹, Kelei Zhao¹

Affiliations

¹ Affiliated Hospital/Clinical College of Chengdu University, Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province, School of Pharmacy, Chengdu University, Chengdu, Sichuan, 610106, People's Republic of China.
² Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, People's Republic of China.

Abstract

Pseudomonas aeruginosa is a notorious Gram-negative opportunistic pathogen that normally causes acute and chronic infections in a wide range of hosts. In this study, a multi-resistant P. aeruginosa isolate L1a harboring an infrequent plasmid with red fluorescence was obtained from the bronchoalveolar lavage fluid of a patient with chronic obstructive pulmonary disease. The results of susceptibility testing and virulence-related phenotypic identification revealed that P. aeruginosa L1a was resistant to levofloxacin, cefepime, aztreonam, and imipenem and showed significantly stronger capacities for swimming and pyocyanin production than the reference strain PAO1. The genome of P. aeruginosa L1a was assembled into one circular chromosome (6,216,913 bp) and one circular plasmid (9111 bp). P. aeruginosa L1a was found to belong to the multilocus sequence type ST549, and serotype O5, and carried 8 drug resistance genes and 18 multidrug efflux pump-related genes in the chromosomal DNA. The plasmid pL1a harbored a tetracycline resistant gene tetA and a functional red fluorescence protein. This study reports a multidrug resistant P. aeruginosa clinical isolate harboring an infrequent red fluorescence plasmid for the first time.

Keywords: Pseudomonas aeruginosa; drug resistance; fluorescence; phenotypic identification; whole-genome sequencing.

Grants and funding

This work was supported by the National Natural Science Foundation of China (31970131), the Open Project of Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province (ARRLKF21-06), the Sichuan Science and Technology Program (2021JDJQ0042), the high-level talent training program of Chengdu University (2081920066), and the innovation foundation of the Affiliated Hospital of Chengdu University (CDFYCX202209).