Acidic Microenvironment-Sensitive Core-Shell Microcubes: The Self-assembled and the Therapeutic Effects for Caries Prevention

Tsai-Miao Shih; Jui-Fu Hsiao; Dar-Bin Shieh; Guochuan Emil Tsai

doi:10.1055/s-0042-1757464

Acidic Microenvironment-Sensitive Core-Shell Microcubes: The Self-assembled and the Therapeutic Effects for Caries Prevention

Eur J Dent. 2023 Jul;17(3):863-870. doi: 10.1055/s-0042-1757464. Epub 2022 Dec 19.

Authors

Tsai-Miao Shih¹, Jui-Fu Hsiao¹, Dar-Bin Shieh^{2

3

4}, Guochuan Emil Tsai^{1

5}

Affiliations

¹ Department of Research and Development, SyneuRx International (Taiwan) Corp., New Taipei City, Taiwan.
² School of Dentistry and Institute of Oral Medicine, National Cheng Kung University, Tainan, Taiwan.
³ Center of Applied Nanomedicine and Core Facility Center, National Cheng Kung University, Tainan, Taiwan.
⁴ Department of Stomatology, National Cheng Kung University Hospital, Tainan, Taiwan.
⁵ Department of Psychiatry and Biobehavioral Sciences, UCLA School of Medicine, Los Angeles, California, United States.

Abstract

Objectives: The aim of this study was to develop a new material with integrated interface design that could achieve the purpose of environmental-sensing controlled release against cariogenic bacteria. Furthermore, this material can rebalance oral flora and serve as a preventive and reparative measure of dental caries.

Materials and methods: NaF@PAA@HA@polyelectrolytes@HA@PAA particles were synthesized using the method of two-solution phases precipitation followed by biocompatible polymers coating layer by layer. The structure of the particles was confirmed by transmission electron microscope. The fluoride release profile was measured by fluoride ion electrode. Antimicrobial activity against the cariogenic microorganisms was analyzed by scanning electron microscopy and energy dispersive spectrum. The efficacy experiments were conducted on tooth enamel slides to evaluated fluoride absorption and antibacterial activity of the prototype toothpaste containing microcube particles RESULTS: The structure of NaF@PAA@HA@polyelectrolytes@HA@PAA particles showed a core surrounded by tooth-adhesion polymer layers in thin fin or filament structure. The loaded concentration of fluoride in the particles' core was 148,996 ± 28,484 ppm. NaF@PAA@HA@polyelectrolytes@HA@PAA particles showed selective inhibition of cariogenic microorganisms over probiotic strains and stronger fluoride adhesion on tooth enamel. A burst release (over 80%) of fluoride from the particle-containing toothpaste was observed under cariogenic acidic environment (pH < 5), while it remained extremely low under neutral environment. Compared with the best results of commercial toothpastes, our prototype toothpaste increased enamel fluoride uptake by 8-fold in normal enamel slides and by 11-fold in the slides with induced white spot lesions after either 1- or 7-day treatment. The prototype toothpaste also showed better inhibition of cariogenic microorganisms than the commercial brands. The coverage area of cariogenic bacteria under our toothpaste treatment was 73% on normal enamel slides compared with the commercial brands, while it was 69% in the induced white spot lesions.

Conclusions: In our study, an intelligent toothpaste was developed that selectively inhibits cariogenic bacteria by microenvironment proton-triggered fluoride release. Such novel design would accomplish a favorable flora balance for optimal long-term oral health.

The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).