Supplementation of quinoa peptides alleviates colorectal cancer and restores gut microbiota in AOM/DSS-treated mice

Xin Fan; Huimin Guo; Cong Teng; Xiushi Yang; Peiyou Qin; Aurore Richel; Lizhen Zhang; Christophe Blecker; Guixing Ren

doi:10.1016/j.foodchem.2022.135196

Supplementation of quinoa peptides alleviates colorectal cancer and restores gut microbiota in AOM/DSS-treated mice

Food Chem. 2023 May 15:408:135196. doi: 10.1016/j.foodchem.2022.135196. Epub 2022 Dec 12.

Authors

Xin Fan¹, Huimin Guo², Cong Teng³, Xiushi Yang³, Peiyou Qin³, Aurore Richel⁴, Lizhen Zhang⁵, Christophe Blecker⁶, Guixing Ren⁷

Affiliations

¹ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China; Department of Food Science and Formulation, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
² Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; Laboratory of Biomass and Green Technologies, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
³ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
⁴ Laboratory of Biomass and Green Technologies, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
⁵ School of Life Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China. Electronic address: lizhen@sxu.edu.cn.
⁶ Department of Food Science and Formulation, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium. Electronic address: christophe.blecker@uliege.be.
⁷ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China; School of Life Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China. Electronic address: renguixing@caas.cn.

PMID: 36535178
DOI: 10.1016/j.foodchem.2022.135196

Abstract

Quinoa protein hydrolysate has been previously reported to exert anti-cancer effects in cultured colon cancer cells. Here, we investigated the effect of quinoa protein and its hydrolysate on an azoxymethane/dextran sulfate sodium (AOM/DSS)-induced mouse model of colorectal cancer (CRC) and examined its underlying mechanism using gut microbiota analysis and short chain fatty acids (SCFAs) production analysis. Our results showed that quinoa protein or its hydrolysate mitigated the clinical symptoms of CRC and increased SCFAs contents in colon tissues. Moreover, administration of quinoa protein or its hydrolysate partially alleviated gut microbiota dysbiosis in CRC mice by decreasing the abundance of pathogenic bacteria and increasing the abundance of probiotics. Additionally, PICRUSt analysis revealed that the functional profile of gut microbiota in the quinoa protein treated groups was more similar to that of the control group. These findings indicated that the modulation of gut microbiota by quinoa protein diet intervention may ameliorate AOM/DSS-induced CRC.

Keywords: Colon cancer; Gastrointestinal digestion; Microbial dysbiosis; Quinoa protein hydrolysate; Short chain fatty acids.

MeSH terms

Animals
Azoxymethane / adverse effects
Chenopodium quinoa*
Colitis* / chemically induced
Colorectal Neoplasms* / chemically induced
Colorectal Neoplasms* / drug therapy
Colorectal Neoplasms* / metabolism
Dextran Sulfate
Disease Models, Animal
Gastrointestinal Microbiome*
Mice
Mice, Inbred C57BL
Peptides / pharmacology
Probiotics*

Substances

Azoxymethane
Dextran Sulfate
Peptides