Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus

Fengling Li; Ji Qi; Zengzheng Ren; Xiaoli Hu; Yan Chen; Bowei Li; Xiuli Fu

doi:10.1016/j.microc.2022.108304

Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus

Microchem J. 2023 Feb:185:108304. doi: 10.1016/j.microc.2022.108304. Epub 2022 Dec 10.

Authors

Fengling Li¹, Ji Qi², Zengzheng Ren¹, Xiaoli Hu¹, Yan Chen³, Bowei Li², Xiuli Fu¹

Affiliations

¹ School of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.
² CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, The Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
³ School of Resources and Environmental Engineering, Shandong Agriculture and Engineering University, Jinan 250100, China.

Abstract

Coronavirus disease 2019 (COVID-19) and influenza A are two respiratory infectious diseases with similar clinical manifestations. Because of the complex global epidemic situation of COVID-19, the distinction and diagnosis of COVID-19 and influenza A infected persons is crucial for epidemic prevention and control. In this study, tetrahedral DNA framework (TDF) was combined with a rotational paper-based analytical device, and the color change generated by the reaction between horseradish peroxidase (HRP) and 3,3'5,5'-tetramethylbenzidine (TMB)-H₂O₂ was used for grayscale signal analysis by ImageJ software. The quantitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A H1N1 virus were realized simultaneously. Under the optimal conditions, the paper-based analytical device showed a good linear relationship between the two viruses in the range of 10^-14-10^-8g/mL, and the two viruses were not affected by cross reaction. This sensor provides a convenient and reliable method for clinical rapid differentiation and diagnosis of COVID-19 and influenza A.

Keywords: Influenza A H1N1 virus; Paper-based analytical device; SARS-CoV-2; Tetrahedral DNA framework.