Crystal violet-based assay for the assessment of bacterial biofilm formation in medical tubing

Marcin Borowicz; Dorota M Krzyżanowska; Sylwia Jafra

doi:10.1016/j.mimet.2022.106656

Crystal violet-based assay for the assessment of bacterial biofilm formation in medical tubing

J Microbiol Methods. 2023 Jan:204:106656. doi: 10.1016/j.mimet.2022.106656. Epub 2022 Dec 14.

Authors

Marcin Borowicz¹, Dorota M Krzyżanowska², Sylwia Jafra³

Affiliations

¹ Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. A. Abrahama 58, 80-307 Gdańsk, Poland.
² Laboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology of the University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. A. Abrahama 58, 80-307 Gdańsk, Poland.
³ Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdańsk and Medical University of Gdańsk, University of Gdańsk, ul. A. Abrahama 58, 80-307 Gdańsk, Poland. Electronic address: sylwia.jafra@ug.edu.pl.

PMID: 36526040
DOI: 10.1016/j.mimet.2022.106656

Abstract

Crystal violet-based assay is widely used to evaluate the early stages of bacterial biofilm formation. Here, we modified it to study biofilm formation in medical tubing. While cost-effective and requiring no sophisticated equipment, our method provided quantitative and qualitative distinctions between the ability of four bacterial strains to colonize PVC catheters.

Keywords: Biofilm; Catheter; Colonization; Escherichia coli; Pseudomonas aeruginosa; Staphylococcus aureus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents
Bacteria
Biofilms
Gentian Violet*
Microbial Sensitivity Tests
Pseudomonas aeruginosa
Staphylococcus aureus*

Substances

Gentian Violet
Anti-Bacterial Agents