Efficient purification of flavonoids from bamboo shoot residues of Phyllostachys edulis by macroporous resin and their hypoglycemic activity

Yanbin Wang; Yalan Zhang; Junwen Cheng; Jiancheng Zhao; Rui Shi; Liang He; Qin Li; Yongjian Chen

doi:10.1016/j.fochx.2022.100505

Efficient purification of flavonoids from bamboo shoot residues of Phyllostachys edulis by macroporous resin and their hypoglycemic activity

Food Chem X. 2022 Nov 12:16:100505. doi: 10.1016/j.fochx.2022.100505. eCollection 2022 Dec 30.

Authors

Yanbin Wang¹, Yalan Zhang^{1

2}, Junwen Cheng¹, Jiancheng Zhao³, Rui Shi², Liang He¹, Qin Li³, Yongjian Chen⁴

Affiliations

¹ The Key Laboratory of Biochemical Utilization of Zhejiang Province, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou 310023, China.
² College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 200093, China.
³ Bamboo Shoots Engineering Research Center of the State Forestry Bureau, Department of Bamboo, Zhejiang Academy of Forestry, Hangzhou 310023, China.
⁴ Zhejiang Shengshi Biotechnology Co., Ltd., Huzhou 313399, China.

Abstract

The adsorption/desorption process of flavonoids obtained from bamboo shoots residues of Phyllostachys edulis (RPEFs) was established by resin screening, adsorption isotherms, adsorption kinetics and dynamic tests. Results indicated the Langmuir equation fairly fitted the isotherms data and the pseudo-second-order model accurately described its kinetic adsorption on HPD-500 resin. The optimal process was as follows: 2 mg/mL of solution, 2.8 BV feed volume under 2 BV/h flow rate for adsorption; 5 BV volume of 70 % ethanol with a flow rate of 2 BV/h for desorption. After enrichment, the flavonoids in RPEFs increased from 1.39 % to 37.34 %. The analysis of UPLC-TOF-MS/MS uncovered that the purified RPEFs were composed of four major flavonoids. It had potent hypoglycemic activity via PI3K/AKT pathway by upregulating the protein expression of PI3K, AKT, IRS-1, and GLUT4 in a dose-dependent manner based on HepG2-IR cells. Those results provide possible utilization of Phyllostachys edulis by-products in food additives.

Keywords: Adsorption kinetics; Bamboo shoots residue; Enrichment; Flavonoids; Hypoglycemic activity.