The complete plastome of Cynanchum rostellatum (Apocynaceae), an indigenous plant in Korea

Sae Hyun Lee; Woojong Jang; Eunbi Kim; Jiseok Kim; Haiguang Gong; Jong-Soo Kang; Hyeonah Shim; Jee Young Park; Tae-Jin Yang

doi:10.1080/23802359.2022.2148489

The complete plastome of Cynanchum rostellatum (Apocynaceae), an indigenous plant in Korea

Mitochondrial DNA B Resour. 2022 Dec 9;7(12):2035-2039. doi: 10.1080/23802359.2022.2148489. eCollection 2022.

Authors

Sae Hyun Lee¹, Woojong Jang^{1

2}, Eunbi Kim¹, Jiseok Kim¹, Haiguang Gong^{1

3}, Jong-Soo Kang¹, Hyeonah Shim¹, Jee Young Park¹, Tae-Jin Yang¹

Affiliations

¹ Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
² National Institute of Horticultural and Herbal Science, RDA, Eumseong, Republic of Korea.
³ Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, PR China.

Abstract

The climbing plant Cynanchum rostellatum (Turcz.) Liede & Khanum is widely distributed throughout Korea and Northeast Asia as a member of the Apocynaceae family. Although this plant has a high value in medicinal and industrial purposes, genetic research on this plant is insufficient. This study announces the complete plastid genome (plastome) sequence of C. rostellatum with 663× mean coverage, which was assembled using 763 Mbp short-read data generated by the Illumina HiSeq X platform. The C. rostellatum plastome was 158,018 bp in length and displayed the typical quadripartite structure composed of the large single-copy (LSC) region (89,058 bp), the small single-copy (SSC) region (18,718 bp), and a pair of inverted repeat (IR) regions (25,116 bp). A total of 129 genes have been annotated, including 84 protein-coding genes, 37 transfer RNA genes, and eight ribosomal RNA genes. Phylogenetic analysis indicated the genus Cynanchum including 12 Cynanchum plastome sequences, was monophyletic and was located within the sub-family Asclepiadoideae. Two C. rostellatum plastomes, including the plastome assembled in this study, formed a subclade and were sister to the C. thesioides plastome, whereas the other C. rostellatum, which was previously reported one, was located within the clade of C. wilfordii and C. bungei.

Keywords: Cynanchum; Cynanchum rostellatum; Plastid genome; phylogenetic analysis.

Grants and funding

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIT) [No. 2020R1A2C3007885]; Global Visiting Fellowship, BrainKorea 21 Four, Seoul National University, Seoul, Korea; “Cooperative Research Program for Agriculture Science and Technology Development [Project No. PJ017134]" Rural Development Administration, Republic of Korea.